|其他摘要||Pinaceae is the largest family of gymnosperms and comprises over 230 species, there are 10 genus 113 species 29 var. species in China, and they are everygreen or deciduous arbores. Some species have been used to treats diseases for thousands years. Many compounds were isolated from Pinaceae, including triterpenes, diterpenes, lignans and flavones. Some of them showed anti-fungus, anti-bacteria, anti-tumor, anti-HIV, anti-oxidant and anti-feedant activities. In searching of some active compounds from Pinaceae, active constituents of three species (Cathaya argyrophylla Chun et Kuang, Pseudolarix kaempferi (Lindl) Gord, Keteleeria evelyniana Mast.) have been investigated on the basis of primary activity test of 18 Pinaceae plants. Fifty seven compounds (including three new ones) have been isolated by means of chromatographic methods. Their structures were elucidated on the basis of spectral (MS, 1D- and 2D-NMR, IR, UV) and chemical methods. These compouds cover monoterpenoids, norsesquiterpenoids, diterpenoids, triterpenoids, lignans and flavonoids. Finally, a mini-review on the triterpenoids constituents of Pinaceae plants was presented in the last chapter. This thesis is composed of three chapters:
Chapter 1 Chemical constituents of three Pinaceae plants
During investigation on chemical constituents of branches of methanol or ethanol extracts of three Pinaceae species (Cathaya argyrophylla, Pseudolarix kaempferi, Keteleeria evelyniana), fifty seven compounds were isolated and identified by spectroscopic analysis. Among them, there are eight triterpenoids, five diterpenoids, twenty lignans and twenty four other constituents, including three new compounds: i.e. (-)-berchemol-9’-acetate (13) from Cathaya argyrophylla; pseudolarioside A (1) and pseudolarioside B (3) from Pseudolarix kaempferi. The characteristic constituents of Pinaceae plants are lignans, flavonoids and triterpenoids, the content of lignans are more than other constituents in Pinaceae plants.
Chapter 2 Studies on bioativities of Pinaceae plants
Different extracts of 95% ethanol, petroleum ether, EtOAc, n-BuOH and H2O of eighteen Pinaceae species (Abies delavayi, Cedrus deodara, Keteleeria fortunei, K. evelyniana, Picea brachytyla var. complanata, P. abies, Cathaya argyophlla, Pseudolarix kaempferi, Pinus halepensis, P. pinea, P. sylvestris, P. rediata, P. pinaster, P. elliottii, P. kesiya var. langbianensis, P. taeda, Tusga chinensis var. robusta, T. dumosa) and some compounds from Keteleeria evelyniana were tested on anti-osteoporosis (CAT-K), metabolism (PP1), anti-thrombus (PAI-1) and anti-tumor (CAT-B and CDC25 ) assays. Results indicated that petroleum ether extract of Pinus halepensis showed activity on PAI-1 assay. some H2O extracts showed activities on CAT-K assay.
Chapter 3 Progress on the triterpenoid constituents of Pinaceae plants
This chapter summarized triterpenoid constituents and their pharmacological activities of Pinaceae plants.|