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Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology
He, Jun1; Zhang, Zhi-Rong1; Yang, Jun-Bo1; Wang, Hong2; Meng, Jing3; Meng,J (reprint author),Yunnan Agr Univ,Coll Hort & Landscape,Kunming 650201,Peoples R China.; mengjing1125@gmail.com
2015-06-01
发表期刊CONSERVATION GENETICS RESOURCES
卷号7期号:2页码:579-581
摘要Aconitum vilmorinianum is a very important Chinese traditional medicinal plant, which is one of the main raw materials in Yunnan Baiyao as an analgesic and anti-inflammatory agent. Recently, it has been threatened by overexploitation and human disturbances. Genetic background of this species is essential for the efficient conservation and rational utilization. Therefore, a total of 18 novel microsatellite markers were developed for A. vilmorinianum using next-generation sequencing technology. The number of alleles per locus ranged from 1 to 5, with a mean of 2.560, and the observed and expected heterozygosities ranged from 0 to 1.000 and from 0 to 0.733, respectively. These polymorphic microsatellite loci will be especially useful for genetics studies of this important medicinal plant.
关键词Aconitum Vilmorinianum Microsatellite Markers Genetics Medicinal Plant
资助信息National Natural Science Foundation of China [31100178]
学科领域Biodiversity Conservation ; Genetics & Heredity
DOI10.1007/s12686-015-0432-8
收录类别SCI
语种英语
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
WOS类目Biodiversity Conservation ; Genetics & Heredity
WOS记录号WOS:000354213300067
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/21362
专题中国西南野生生物种质资源库
通讯作者Meng,J (reprint author),Yunnan Agr Univ,Coll Hort & Landscape,Kunming 650201,Peoples R China.; mengjing1125@gmail.com
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
3.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Peoples R China
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He, Jun,Zhang, Zhi-Rong,Yang, Jun-Bo,et al. Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology[J]. CONSERVATION GENETICS RESOURCES,2015,7(2):579-581.
APA He, Jun.,Zhang, Zhi-Rong.,Yang, Jun-Bo.,Wang, Hong.,Meng, Jing.,...&mengjing1125@gmail.com.(2015).Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology.CONSERVATION GENETICS RESOURCES,7(2),579-581.
MLA He, Jun,et al."Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology".CONSERVATION GENETICS RESOURCES 7.2(2015):579-581.
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