| Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau | |
Yang, Yunqiang1,2 ; Li, Xiong1,2,3; Kong, Xiangxiang1,2; Ma, Lan1,2,3; Hu, Xiangyang1,2; Yang, Yongping1,2; Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Divers & Biogeog East Asia,Kunming 650204,Peoples R China.; yangyp@mail.kib.ac.cn
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| 2015-05-01 | |
| 发表期刊 | FUNCTIONAL & INTEGRATIVE GENOMICS
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| 卷号 | 15期号:3页码:295-307 |
| 摘要 | Natural selection drives species adaptations to biotic and abiotic stresses. Species distributed along a moisture gradient, such as Stipa purpurea, a dominant grass in alpine arid and semi-arid meadows on the Tibetan Plateau, provide an opportunity to evaluate the effects of long-term adaptation to differing degrees of drought stress on gene expression. However, the genetic basis of this divergence remains largely unknown. Next-generation sequencing technologies have provided important genome-wide insights on the evolution of organisms for which genomic information is lacking. To understand how S. purpurea responds to drought stress, we selected five populations distributed along the degressive rainfall line on the northwestern Tibetan Plateau that currently present evolutionary acclimation to localized drought pressure at the physiological and biochemical levels and compared their transcriptome responses. In addition, we performed de novo assembly of the S. purpurea transcriptome using short read sequencing technology and successfully assembled 84,298 unigenes from approximately 51 million sequencing reads. We quantified gene expression level to compare their transcriptome responses using mRNA-Seq and identified differentially expressed transcripts that are involved in primary and secondary plant metabolism, plant hormone synthesis, defense responses, and cell wall synthesis. Furthermore, physiological and biochemical evidence supports that abscisic acid (ABA) accumulation and cell wall strengthening derived from the differential transcripts contribute to the tolerance of S. purpurea to drought stress. The mechanisms by which S. purpurea adapts to drought stress provide new insight into how plants ecologically adapt and evolve. |
| 关键词 | Transcriptome Stipa Purpurea Gradient Drought Tibetan Plateau |
| 学科领域 | Genetics & Heredity |
| DOI | 10.1007/s10142-014-0419-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353232500004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/20656 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 通讯作者 | Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Divers & Biogeog East Asia,Kunming 650204,Peoples R China.; yangyp@mail.kib.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yunqiang,Li, Xiong,Kong, Xiangxiang,et al. Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2015,15(3):295-307. |
| APA | Yang, Yunqiang.,Li, Xiong.,Kong, Xiangxiang.,Ma, Lan.,Hu, Xiangyang.,...&yangyp@mail.kib.ac.cn.(2015).Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau.FUNCTIONAL & INTEGRATIVE GENOMICS,15(3),295-307. |
| MLA | Yang, Yunqiang,et al."Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau".FUNCTIONAL & INTEGRATIVE GENOMICS 15.3(2015):295-307. |
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