Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau
Yang, Yunqiang1,2; Li, Xiong1,2,3; Kong, Xiangxiang1,2; Ma, Lan1,2,3; Hu, Xiangyang1,2; Yang, Yongping1,2; Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Divers & Biogeog East Asia,Kunming 650204,Peoples R China.; yangyp@mail.kib.ac.cn
2015-05-01
发表期刊FUNCTIONAL & INTEGRATIVE GENOMICS
卷号15期号:3页码:295-307
摘要Natural selection drives species adaptations to biotic and abiotic stresses. Species distributed along a moisture gradient, such as Stipa purpurea, a dominant grass in alpine arid and semi-arid meadows on the Tibetan Plateau, provide an opportunity to evaluate the effects of long-term adaptation to differing degrees of drought stress on gene expression. However, the genetic basis of this divergence remains largely unknown. Next-generation sequencing technologies have provided important genome-wide insights on the evolution of organisms for which genomic information is lacking. To understand how S. purpurea responds to drought stress, we selected five populations distributed along the degressive rainfall line on the northwestern Tibetan Plateau that currently present evolutionary acclimation to localized drought pressure at the physiological and biochemical levels and compared their transcriptome responses. In addition, we performed de novo assembly of the S. purpurea transcriptome using short read sequencing technology and successfully assembled 84,298 unigenes from approximately 51 million sequencing reads. We quantified gene expression level to compare their transcriptome responses using mRNA-Seq and identified differentially expressed transcripts that are involved in primary and secondary plant metabolism, plant hormone synthesis, defense responses, and cell wall synthesis. Furthermore, physiological and biochemical evidence supports that abscisic acid (ABA) accumulation and cell wall strengthening derived from the differential transcripts contribute to the tolerance of S. purpurea to drought stress. The mechanisms by which S. purpurea adapts to drought stress provide new insight into how plants ecologically adapt and evolve.
关键词Transcriptome Stipa Purpurea Gradient Drought Tibetan Plateau
资助信息National Natural Science Foundation of China (NSFC) [41271058]; Major State Basic Research Development Program of China [2010CB951704]
学科领域Genetics & Heredity
DOI10.1007/s10142-014-0419-7
收录类别SCI
语种英语
WOS研究方向Genetics & Heredity
WOS类目Genetics & Heredity
WOS记录号WOS:000353232500004
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20656
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Divers & Biogeog East Asia,Kunming 650204,Peoples R China.; yangyp@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yang, Yunqiang,Li, Xiong,Kong, Xiangxiang,et al. Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2015,15(3):295-307.
APA Yang, Yunqiang.,Li, Xiong.,Kong, Xiangxiang.,Ma, Lan.,Hu, Xiangyang.,...&yangyp@mail.kib.ac.cn.(2015).Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau.FUNCTIONAL & INTEGRATIVE GENOMICS,15(3),295-307.
MLA Yang, Yunqiang,et al."Transcriptome analysis reveals diversified adaptation of Stipa purpurea along a drought gradient on the Tibetan Plateau".FUNCTIONAL & INTEGRATIVE GENOMICS 15.3(2015):295-307.
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