Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient

doi:10.1371/journal.pone.0117475

	Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient
	Yang, Yunqiang1,2,3 ; Dong, Chao 1,2,3,5; Yang, Shihai 4,5; Li, Xiong1,2,3,5 ; Sun, Xudong 1,2,3; Yang, Yongping1,2,3 ; Sun,XD (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Div & Biogeog East Asia,Kunming,Peoples R China.; sunxudong@mail.kib.ac.cn ; yangyp@mail.kib.ac.cn
	2015-02-03
发表期刊	PLOS ONE
卷号	10 期号:2 页码:e0117475
摘要	Stipa purpurea, an endemic forage species on the Tibetan Plateau, is highly resistant to cold and drought, but the mechanisms underlying its responses to drought stress remain elusive. An understanding of such mechanisms may be useful for developing cultivars that are adaptable to water deficit. In this study, we analyzed the physiological and proteomic responses of S. purpurea under increasing drought stress. Seedlings of S. purpurea were subjected to a drought gradient in a controlled experiment, and proteins showing changes in abundance under these conditions were identified by two-dimensional electrophoresis followed by mass spectrometry analysis. A western blotting analysis was conducted to confirm the increased abundance of a heat-shock protein, NCED2, and a dehydrin in S. purpurea seedlings under drought conditions. We detected carbonylated proteins to identify oxidation-sensitive proteins in S. purpurea seedlings, and found that ribulose-1, 5-bisphosphate carboxylase oxygenase (RuBisCO) was one of the oxidation-sensitive proteins under drought. Together, these results indicated drought stress might inhibit photosynthesis in S. purpurea by oxidizing RuBisCO, but the plants were able to maintain photosynthetic efficiency by a compensatory upregulation of unoxidized RuBisCO and other photosynthesis-related proteins. Further analyses confirmed that increased abundance of antioxidant enzymes could balance the redox status of the plants to mitigate drought induced oxidative damage.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0117475
收录类别	SCI
语种	英语
WOS记录号	WOS:000348822600071
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/20655
专题	中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Sun,XD (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Div & Biogeog East Asia,Kunming,Peoples R China.; sunxudong@mail.kib.ac.cn; yangyp@mail.kib.ac.cn
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming, Peoples R China 4.Chinese Acad Sci, Key Lab Tibetan Environm Changes & Land Surface P, Inst Tibetan Plateau Res, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yunqiang,Dong, Chao,Yang, Shihai,et al. Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient[J]. PLOS ONE,2015,10(2):e0117475.
APA	Yang, Yunqiang.,Dong, Chao.,Yang, Shihai.,Li, Xiong.,Sun, Xudong.,...&yangyp@mail.kib.ac.cn.(2015).Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient.PLOS ONE,10(2),e0117475.
MLA	Yang, Yunqiang,et al."Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient".PLOS ONE 10.2(2015):e0117475.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Yang-2015-Physiologi（6656KB）			开放获取	CC BY-NC-ND	浏览下载