Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient
Yang, Yunqiang1,2,3; Dong, Chao1,2,3,5; Yang, Shihai4,5; Li, Xiong1,2,3,5; Sun, Xudong1,2,3; Yang, Yongping1,2,3; Sun,XD (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Div & Biogeog East Asia,Kunming,Peoples R China.; sunxudong@mail.kib.ac.cn; yangyp@mail.kib.ac.cn
2015-02-03
发表期刊PLOS ONE
卷号10期号:2页码:e0117475
摘要Stipa purpurea, an endemic forage species on the Tibetan Plateau, is highly resistant to cold and drought, but the mechanisms underlying its responses to drought stress remain elusive. An understanding of such mechanisms may be useful for developing cultivars that are adaptable to water deficit. In this study, we analyzed the physiological and proteomic responses of S. purpurea under increasing drought stress. Seedlings of S. purpurea were subjected to a drought gradient in a controlled experiment, and proteins showing changes in abundance under these conditions were identified by two-dimensional electrophoresis followed by mass spectrometry analysis. A western blotting analysis was conducted to confirm the increased abundance of a heat-shock protein, NCED2, and a dehydrin in S. purpurea seedlings under drought conditions. We detected carbonylated proteins to identify oxidation-sensitive proteins in S. purpurea seedlings, and found that ribulose-1, 5-bisphosphate carboxylase oxygenase (RuBisCO) was one of the oxidation-sensitive proteins under drought. Together, these results indicated drought stress might inhibit photosynthesis in S. purpurea by oxidizing RuBisCO, but the plants were able to maintain photosynthetic efficiency by a compensatory upregulation of unoxidized RuBisCO and other photosynthesis-related proteins. Further analyses confirmed that increased abundance of antioxidant enzymes could balance the redox status of the plants to mitigate drought induced oxidative damage.
资助信息National Natural Science Foundation of China (NSFC) [41271058]; Major State Basic Research Development Program of China [2010CB951704]
学科领域Multidisciplinary Sciences
DOI10.1371/journal.pone.0117475
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000348822600071
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20655
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Sun,XD (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Div & Biogeog East Asia,Kunming,Peoples R China.; sunxudong@mail.kib.ac.cn; yangyp@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming, Peoples R China
4.Chinese Acad Sci, Key Lab Tibetan Environm Changes & Land Surface P, Inst Tibetan Plateau Res, Beijing, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
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Yang, Yunqiang,Dong, Chao,Yang, Shihai,et al. Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient[J]. PLOS ONE,2015,10(2):e0117475.
APA Yang, Yunqiang.,Dong, Chao.,Yang, Shihai.,Li, Xiong.,Sun, Xudong.,...&yangyp@mail.kib.ac.cn.(2015).Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient.PLOS ONE,10(2),e0117475.
MLA Yang, Yunqiang,et al."Physiological and Proteomic Adaptation of the Alpine Grass Stipa purpurea to a Drought Gradient".PLOS ONE 10.2(2015):e0117475.
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