| Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia | |
Xu,Songzhi; Li,Dezhu ; Li,Jianwu; Xiang,Xiaoguo; Jin,Weitao; Huang,Weichang; Jin,Xiaohua; Huang,Luqi; Jin,XH (reprint author),Chinese Acad Sci,Inst Bot,State Key Lab Systemat & Evolutionary Bot,Beijing 100093,Peoples R China.; xiaohuajin@ibcas.ac.cn; huangluqi01@126.com
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| 2015 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 10期号:1页码:e0115168 |
| 摘要 | DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera. |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1371/journal.pone.0115168 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348203500009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/20653 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 通讯作者 | Jin,XH (reprint author),Chinese Acad Sci,Inst Bot,State Key Lab Systemat & Evolutionary Bot,Beijing 100093,Peoples R China.; xiaohuajin@ibcas.ac.cn; huangluqi01@126.com |
| 推荐引用方式 GB/T 7714 | Xu,Songzhi,Li,Dezhu,Li,Jianwu,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1):e0115168. |
| APA | Xu,Songzhi.,Li,Dezhu.,Li,Jianwu.,Xiang,Xiaoguo.,Jin,Weitao.,...&huangluqi01@126.com.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1),e0115168. |
| MLA | Xu,Songzhi,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015):e0115168. |
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