Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia
Xu,Songzhi; Li,Dezhu; Li,Jianwu; Xiang,Xiaoguo; Jin,Weitao; Huang,Weichang; Jin,Xiaohua; Huang,Luqi; Jin,XH (reprint author),Chinese Acad Sci,Inst Bot,State Key Lab Systemat & Evolutionary Bot,Beijing 100093,Peoples R China.; xiaohuajin@ibcas.ac.cn; huangluqi01@126.com
2015
发表期刊PLOS ONE
卷号10期号:1页码:e0115168
摘要DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera.
资助信息National Natural Science Foundation of China [31107176, 31470299, J1310002]; Chinese Special Fund for Medicine Research in the Public Interest [201407003]
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20653
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Jin,XH (reprint author),Chinese Acad Sci,Inst Bot,State Key Lab Systemat & Evolutionary Bot,Beijing 100093,Peoples R China.; xiaohuajin@ibcas.ac.cn; huangluqi01@126.com
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Xu,Songzhi,Li,Dezhu,Li,Jianwu,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1):e0115168.
APA Xu,Songzhi.,Li,Dezhu.,Li,Jianwu.,Xiang,Xiaoguo.,Jin,Weitao.,...&huangluqi01@126.com.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1),e0115168.
MLA Xu,Songzhi,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015):e0115168.
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