Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses

doi:10.1073/pnas.0702398104

	Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses
	Li, Yanmei ; Wang, Lihua ; Li, Shunlin; Chen, Xiaoying ; Shen, Yuemao ; Zhang, Zhongkai ; He, Hongping ; Xu, Wenbo ; Shu, Yuelong ; Liang, Guodong ; Fang, Rongxiang ; Hao, Xiaojiang
	2007-05-08
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	104 期号:19 页码:8083-8088
摘要	Plants have evolved multiple mechanisms to selectively suppress pathogens by production of secondary metabolites with antimicrobial activities. Therefore, direct selections for antiviral compounds from plants can be used to identify new agents with potent antiviral activity but not toxic to hosts. Here, we provide evidence that a class of compounds, seco-pregnane steroid glaucogenin C and its monosugar-glycoside cynatratoside A of Strobilanthes cusia and three new pantasugar-glycosides of glaucogenin C of Cynanchum paniculatum, are effective and selective inhibitors to alphavirus-like positive-strand RNA viruses including plant-infecting tobacco mosaic virus (TMV) and animal-infecting Sindbis virus (SINV), eastern equine encephalitis virus, and Getah virus, but not to other RNA or DNA viruses, yet they were not toxic to host cells. In vivo administration of the compounds protected BALB/c mice from lethal SINV infection without adverse effects on the mice. Using TMV and SINV as models, studies on the action mechanism revealed that the compounds predominantly suppress the expression of viral subgenomic RNA(s) without affecting the accumulation of viral genomic RNA. Our work suggested that the viral subgenomic RNA could be a new target for the discovery of antiviral drugs, and that seco-pregnane steroid and its four glycosides found in the two medicinal herbs have the potential for further development as antiviral agents against alphavirus-like positive-strand RNA viruses.
关键词	Antiviral Drugs Strobilanthes Cusia Cynanchum Atratum
学科领域	Multidisciplinary Sciences
DOI	10.1073/pnas.0702398104
收录类别	SCI
语种	英语
WOS记录号	WOS:000246461500063
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2061
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Chinese Acad Sci, State Key Lab Plant Plant Genom, Beijing 100080, Peoples R China 3.Chinese Ctr Dis Control & Prevent, Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100052, Peoples R China 4.Yunnan Acad Agr Sci, Lab Plant Virol, Kunming 650223, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yanmei,Wang, Lihua,Li, Shunlin,et al. Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2007,104(19):8083-8088.
APA	Li, Yanmei.,Wang, Lihua.,Li, Shunlin.,Chen, Xiaoying.,Shen, Yuemao.,...&Hao, Xiaojiang.(2007).Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,104(19),8083-8088.
MLA	Li, Yanmei,et al."Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104.19(2007):8083-8088.

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