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题名: Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus
作者: Wang, Xiaoyan1, 2; Zhang, Xuemei1; Zhao, Lei1; null(郭振华)1
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词: Dendrocalamus latiflorus ; floral induction ; early floral development ; morphological characteristics ; gene expression profiling ; molecular marker
英文摘要: The mechanism of floral transition in bamboo remains unclear. Dendrocalamus latiflorus (Bambusease, Bambusoideae, Poaceae) is an economically and ecologically important clumping bamboo in tropical and subtropical areas. We evaluated morphological characteristics and gene expression profiling to study floral induction and early flower development in D. latiflorus. The detailed morphological studies on vegetative buds and floral organography were completed using paraffin sectioning and scanning electron microscopy. The 3 mm floral buds commence the development of stamen primordia and pistil primordium. Furthermore, homologs of floral transition-related genes, including AP1, TFL1, RFL, PpMADS1, PpMADS2, SPL9, FT, ID1, FCA, and EMF2, were detected and quantified by reverse transcriptase PCR and real-time PCR in vegetative and floral buds, respectively. Distinct expression profiles of ten putative floral initiation homologues that corresponded to the developmental stages defined by bud length were obtained and genes were characterized. Six of the genes (including DlTFL1, DlRFL, DlMADS2, DlID1, DlFCA, DlEMF2) showed statistically significant changes in expression during floral transition. DlAP1 demonstrated a sustained downward trend and could serve as a good molecular marker during floral transition in D. latiflorus. The combined analysis provided key candidate markers to track the transition from the vegetative to reproductive phase.
出版日期: 2014-07-01
卷号: 15, 期号:7, 页码:12074-12093
语种: 英语
收录类别: SCI
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/20469
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, China Southwest Germplasm Bank Wild Species, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China

Recommended Citation:
Wang, Xiaoyan,Zhang, Xuemei,Zhao, Lei,et al. Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(7):12074-12093.
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