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Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus
Wang, Xiaoyan1,2; Zhang, Xuemei1; Zhao, Lei1; Guo, Zhenhua1
2014-07-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume15Issue:7Pages:12074-12093
AbstractThe mechanism of floral transition in bamboo remains unclear. Dendrocalamus latiflorus (Bambusease, Bambusoideae, Poaceae) is an economically and ecologically important clumping bamboo in tropical and subtropical areas. We evaluated morphological characteristics and gene expression profiling to study floral induction and early flower development in D. latiflorus. The detailed morphological studies on vegetative buds and floral organography were completed using paraffin sectioning and scanning electron microscopy. The 3 mm floral buds commence the development of stamen primordia and pistil primordium. Furthermore, homologs of floral transition-related genes, including AP1, TFL1, RFL, PpMADS1, PpMADS2, SPL9, FT, ID1, FCA, and EMF2, were detected and quantified by reverse transcriptase PCR and real-time PCR in vegetative and floral buds, respectively. Distinct expression profiles of ten putative floral initiation homologues that corresponded to the developmental stages defined by bud length were obtained and genes were characterized. Six of the genes (including DlTFL1, DlRFL, DlMADS2, DlID1, DlFCA, DlEMF2) showed statistically significant changes in expression during floral transition. DlAP1 demonstrated a sustained downward trend and could serve as a good molecular marker during floral transition in D. latiflorus. The combined analysis provided key candidate markers to track the transition from the vegetative to reproductive phase.
KeywordDendrocalamus Latiflorus Floral Induction Early Floral Development Morphological Characteristics Gene Expression Profiling Molecular Marker
DOI10.3390/ijms150712074
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000340038500054
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/20469
Collection中国西南野生生物种质资源库
Affiliation1.Chinese Acad Sci, China Southwest Germplasm Bank Wild Species, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China
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Wang, Xiaoyan,Zhang, Xuemei,Zhao, Lei,et al. Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(7):12074-12093.
APA Wang, Xiaoyan,Zhang, Xuemei,Zhao, Lei,&Guo, Zhenhua.(2014).Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,15(7),12074-12093.
MLA Wang, Xiaoyan,et al."Morphology and Quantitative Monitoring of Gene Expression Patterns during Floral Induction and Early Flower Development in Dendrocalamus latiflorus".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 15.7(2014):12074-12093.
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