Hu,XY (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Plant Divers & Biogeog East Asia,Kunming 650201,Peoples R China.
The establishment of ad-abaxial polarity is an important characteristic of the development of lateral organs in plants. YABBY genes encode higher plant-specific nuclear proteins which play critical roles in promoting abaxial cell fate. IaYABBY2 (IaYAB2, Genbank accession no. KF250432), isolated from Incarvillea arguta, is a member of YABBY gene family. Sequence characterization and phylogenetic analyses show that IaYABBY2 is a member of the YAB2 subfamily of Arabidopsis thaliana. Subcellular localization analysis indicates that IaYABBY2 is localized in the nucleus. Ectopic expression of IaYABBY2 in Arabidopsis plants resulted in the partial abaxialization of adaxial epidermises of leaves and sepals and development defect of florescence. The transgenic lines also showed higher level of anthocyanin content and photosynthesis capability after differential environment stress. These results indicate that the IaYABBY2 functions in the ad-abaxial polarity, development of shoot apical meristem, and environmental stress.
Ministry of Science and Technology of China [2012ZX09301002-003001006]
; National Natural Science Foundation of China [81422054, 81373306, 81202441]
; Guangdong Natural Science Funds for Distinguished Young Scholar [S2013050014287]
; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
; high-performance computing platform of Jinan University
1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Sun,Xudong;Guan,Yanlong;Hu,Xiangyang.Isolation and Characterization of IaYABBY2 Gene from Incarvillea arguta,PLANT MOLECULAR BIOLOGY REPORTER,2014,4(97):54144-54148