Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique
Zhou, Jing1,2; Wang, Wencai3; Liu, Mengqi4; Liu, Zhenwen5; Liu,ZW (reprint author),Chinese Acad Sci,Kunming Inst Bot,Lanhei Rd 132,Kunming 650201,Peoples R China.
2014-10-01
发表期刊PHARMACOGNOSY MAGAZINE
ISSN0973-1296
卷号10期号:40页码:385-390
产权排序第三
摘要Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimuras 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.
关键词Apiaceae Dna Barcoding Identification Nrdna Its Peucedanumpraeruptorum l
资助信息National NSFC [31300569]; "Hundreds of Talents" program of the Chinese Academy of Sciences; National NSFC [31300569]; "Hundreds of Talents" program of the Chinese Academy of Sciences
学科领域Chemistry, Medicinal
DOI10.4103/0973-1296.141754
收录类别SCI
语种英语
WOS研究方向Pharmacology & Pharmacy
WOS类目Chemistry, Medicinal
WOS记录号WOS:000344498300001
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18451
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Liu,ZW (reprint author),Chinese Acad Sci,Kunming Inst Bot,Lanhei Rd 132,Kunming 650201,Peoples R China.
作者单位1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China
2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
4.Henan Univ Tradit Chinese Med, Zhengzhou 450046, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Peoples R China
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Zhou, Jing,Wang, Wencai,Liu, Mengqi,et al. Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique[J]. PHARMACOGNOSY MAGAZINE,2014,10(40):385-390.
APA Zhou, Jing,Wang, Wencai,Liu, Mengqi,Liu, Zhenwen,&Liu,ZW .(2014).Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique.PHARMACOGNOSY MAGAZINE,10(40),385-390.
MLA Zhou, Jing,et al."Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique".PHARMACOGNOSY MAGAZINE 10.40(2014):385-390.
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