Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimuras 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.
1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China 2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China 4.Henan Univ Tradit Chinese Med, Zhengzhou 450046, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Peoples R China
Zhou, Jing,Wang, Wencai,Liu, Mengqi,et al. Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique[J]. PHARMACOGNOSY MAGAZINE,2014,10(40):385-390.