中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 中国科学院东亚植物多样性与生物地理学重点实验室  > 期刊论文
题名: Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique
作者: Zhou, Jing1, 2; Wang, Wencai3; Liu, Mengqi4; Liu, Zhenwen5
刊名: PHARMACOGNOSY MAGAZINE
出版日期: 2014-10-01
卷号: 10, 期号:40, 页码:385-390
关键词: Apiaceae ; DNA barcoding ; identification ; nrDNA ITS ; Peucedanumpraeruptorum L
学科分类: Chemistry, Medicinal
DOI: 10.4103/0973-1296.141754
通讯作者: Liu,ZW (reprint author),Chinese Acad Sci,Kunming Inst Bot,Lanhei Rd 132,Kunming 650201,Peoples R China.
文章类型: Article
英文摘要: Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimuras 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.
类目[WOS]: Chemistry, Medicinal
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: INTERNAL TRANSCRIBED SPACER ; NUCLEAR RIBOSOMAL DNA ; SEQUENCE-ANALYSIS ; APIACEAE ; RDNA ; IDENTIFICATION ; PHYLOGENY ; ANGELICA ; MARKERS ; REGION
收录类别: SCI
项目资助者: National NSFC [31300569] ; "Hundreds of Talents" program of the Chinese Academy of Sciences
语种: 英语
WOS记录号: WOS:000344498300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18451
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
liu2014.pdf(908KB)期刊论文出版稿开放获取View Download

作者单位: 1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China
2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
4.Henan Univ Tradit Chinese Med, Zhengzhou 450046, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Peoples R China

Recommended Citation:
Zhou, Jing,Wang, Wencai,Liu, Mengqi,et al. Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA - barcoding technique[J]. PHARMACOGNOSY MAGAZINE,2014,10(40):385-390.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Jing]'s Articles
[Wang, Wencai]'s Articles
[Liu, Mengqi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Jing]‘s Articles
[Wang, Wencai]‘s Articles
[Liu, Mengqi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: liu2014.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace