Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia
Deng, Yuan-Yuan1,2,3; Yi, Yang2; Zhang, Li-Fang2; Zhang, Rui-Fen2; Zhang, Yan2; Wei, Zhen-Cheng2; Tang, Xiao-Jun2; Zhang, Ming-Wei2; Zhang,MW (reprint author),Guangdong Acad Agr Sci,Sericulture & Agri Food Res Inst,Guangzhou 510610,Guangdong,Peoples R China.; yuanyuan_deng@yeah.net; yiyang1986@msn.com; zlf6662@126.com; ruifenzhang@163.com; zhang__yan_@126.com; zhencheng_wei@163.com; xjtang66@163.com; mwzhh@vip.tom.com
2014-09-01
Source PublicationMOLECULES
ISSN1420-3049
Volume19Issue:9Pages:13432-13447
Contribution Rank第一
AbstractMomordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg.kg(-).d(-1) of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55 x 10(4) Da and 4.41 x 10(5) Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
KeywordMomordica Charantia Polysaccharide Immunomodulatory Activity Structure
Subject AreaChemistry
DOI10.3390/molecules190913432
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Organic
WOS IDWOS:000343093100034
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18372
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorZhang,MW (reprint author),Guangdong Acad Agr Sci,Sericulture & Agri Food Res Inst,Guangzhou 510610,Guangdong,Peoples R China.; yuanyuan_deng@yeah.net; yiyang1986@msn.com; zlf6662@126.com; ruifenzhang@163.com; zhang__yan_@126.com; zhencheng_wei@163.com; xjtang66@163.com; mwzhh@vip.tom.com
Affiliation1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Deng, Yuan-Yuan,Yi, Yang,Zhang, Li-Fang,et al. Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia[J]. MOLECULES,2014,19(9):13432-13447.
APA Deng, Yuan-Yuan.,Yi, Yang.,Zhang, Li-Fang.,Zhang, Rui-Fen.,Zhang, Yan.,...&mwzhh@vip.tom.com.(2014).Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia.MOLECULES,19(9),13432-13447.
MLA Deng, Yuan-Yuan,et al."Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia".MOLECULES 19.9(2014):13432-13447.
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