Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia
Deng, Yuan-Yuan1,2,3; Yi, Yang2; Zhang, Li-Fang2; Zhang, Rui-Fen2; Zhang, Yan2; Wei, Zhen-Cheng2; Tang, Xiao-Jun2; Zhang, Ming-Wei2; Zhang,MW (reprint author),Guangdong Acad Agr Sci,Sericulture & Agri Food Res Inst,Guangzhou 510610,Guangdong,Peoples R China.; yuanyuan_deng@yeah.net; yiyang1986@msn.com; zlf6662@126.com; ruifenzhang@163.com; zhang__yan_@126.com; zhencheng_wei@163.com; xjtang66@163.com; mwzhh@vip.tom.com
2014-09-01
发表期刊MOLECULES
ISSN1420-3049
卷号19期号:9页码:13432-13447
产权排序第一
摘要Momordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg.kg(-).d(-1) of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55 x 10(4) Da and 4.41 x 10(5) Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
关键词Momordica Charantia Polysaccharide Immunomodulatory Activity Structure
资助信息National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10, 2012BAD31B03]; National Sparkle Project [2013GA780062]; International Science & Technology Cooperation Program of Guangzhou [2013J4500023]
学科领域Chemistry
DOI10.3390/molecules190913432
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Organic
WOS记录号WOS:000343093100034
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18372
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Zhang,MW (reprint author),Guangdong Acad Agr Sci,Sericulture & Agri Food Res Inst,Guangzhou 510610,Guangdong,Peoples R China.; yuanyuan_deng@yeah.net; yiyang1986@msn.com; zlf6662@126.com; ruifenzhang@163.com; zhang__yan_@126.com; zhencheng_wei@163.com; xjtang66@163.com; mwzhh@vip.tom.com
作者单位1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Deng, Yuan-Yuan,Yi, Yang,Zhang, Li-Fang,et al. Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia[J]. MOLECULES,2014,19(9):13432-13447.
APA Deng, Yuan-Yuan.,Yi, Yang.,Zhang, Li-Fang.,Zhang, Rui-Fen.,Zhang, Yan.,...&mwzhh@vip.tom.com.(2014).Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia.MOLECULES,19(9),13432-13447.
MLA Deng, Yuan-Yuan,et al."Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia".MOLECULES 19.9(2014):13432-13447.
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