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题名: Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia
作者: Deng, Yuan-Yuan1, 2, 3; Yi, Yang2; Zhang, Li-Fang2; Zhang, Rui-Fen2; Zhang, Yan2; Wei, Zhen-Cheng2; Tang, Xiao-Jun2; Zhang, Ming-Wei2
刊名: MOLECULES
关键词: Momordica charantia ; polysaccharide ; immunomodulatory activity ; structure
英文摘要: Momordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg.kg(-).d(-1) of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55 x 10(4) Da and 4.41 x 10(5) Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
出版日期: 2014-09-01
卷号: 19, 期号:9, 页码:13432-13447
DOI标识: 10.3390/molecules190913432
语种: 英语
收录类别: SCI
学科分类: Chemistry
ISSN号: 1420-3049
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18372
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Deng,Yuan-Yuan;Yi,Yang;Zhang,Li-Fang;Zhang,Rui-Fen;Zhang,Yan;Wei,Zhen-Cheng;Tang,Xiao-Jun;Zhang,Ming-Wei.Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia,MOLECULES,2014,19(9):13432-13447
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