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CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION
Lin, Liang1,2; Xu, Qian1; Ma, Junchao1; Yuan, Bin1; Li, Weiqi1; Li,WQ (reprint author),Chinese Acad Sci,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Peoples R China.; weiqili@mail.kib.ac.cn
2014-03-01
发表期刊CRYOLETTERS
ISSN0143-2044
卷号35期号:2页码:129-137
摘要BACKGROUND: Evaluation of the conservation Status of Calanthe plants in China indicated that 18 species were endangered or critically endangered. Comprehensive conservation solutions including ex situ methods, are urgently required to protect Calanthe species in China. OBJECTIVE: The study aims to develop a simple and efficient cryopreservation protocol using droplet-vitrification for Calanthe davidii. METHODS: Protocorm-like bodies (PLBs) were induced from nodal sections of in vitro shoots, and their proliferation was promoted by a thin cell layer culture procedure. Shoot tips excised from three leaf-stage PLBs were used in cryopreservation experiments. Key factors of the droplet-vitrification procedure including sucrose preculture, treatment with PVS2 solution and post-rewarming culture conditions were optimized to achieve a high level of regeneration. RESULTS: When the optimized procedure was applied, 77.8 +/- 3.9% of cryopreserved shoot tips withstood liquid nitrogen exposure and regenerated into new PLBs. CONCLUSION: These results highlighted the importance of post-rewarming osmo-conditioning for regeneration of cryopreserved shoot tips.
关键词Orchidaceae Calanthe Davidii Sucrose Preculture Thin Cell Layer Culture Post-thawing Osmo-conditioning
资助信息National Natural Science Foundation of China [31070262];Fund of the State Key Laboratory of Phytocheinistry and Plant Resources in West China [0807B01211, 097C1211Z1];Kunming Institute of Botany [KSCX2-EW-J-24];Germplasm Bank of Wild Species;CAS Innovation Program of Kunming Institute [540806321211];100-Talents Program of CAS
收录类别SCI
语种英语
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Physiology
WOS类目Biology ; Physiology
WOS记录号WOS:000337718900008
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18223
专题中国西南野生生物种质资源库
通讯作者Li,WQ (reprint author),Chinese Acad Sci,Kunming Inst Bot,Germplasm Bank Wild Species,Kunming 650201,Peoples R China.; weiqili@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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GB/T 7714
Lin, Liang,Xu, Qian,Ma, Junchao,et al. CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION[J]. CRYOLETTERS,2014,35(2):129-137.
APA Lin, Liang.,Xu, Qian.,Ma, Junchao.,Yuan, Bin.,Li, Weiqi.,...&weiqili@mail.kib.ac.cn.(2014).CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION.CRYOLETTERS,35(2),129-137.
MLA Lin, Liang,et al."CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION".CRYOLETTERS 35.2(2014):129-137.
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