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题名: CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION
作者: Lin, Liang1, 2; Xu, Qian1; Ma, Junchao1; Yuan, Bin1; null(李唯奇)1
刊名: CRYOLETTERS
关键词: Orchidaceae ; Calanthe davidii ; sucrose preculture ; thin cell layer culture ; post-thawing osmo-conditioning
英文摘要: BACKGROUND: Evaluation of the conservation Status of Calanthe plants in China indicated that 18 species were endangered or critically endangered. Comprehensive conservation solutions including ex situ methods, are urgently required to protect Calanthe species in China. OBJECTIVE: The study aims to develop a simple and efficient cryopreservation protocol using droplet-vitrification for Calanthe davidii. METHODS: Protocorm-like bodies (PLBs) were induced from nodal sections of in vitro shoots, and their proliferation was promoted by a thin cell layer culture procedure. Shoot tips excised from three leaf-stage PLBs were used in cryopreservation experiments. Key factors of the droplet-vitrification procedure including sucrose preculture, treatment with PVS2 solution and post-rewarming culture conditions were optimized to achieve a high level of regeneration. RESULTS: When the optimized procedure was applied, 77.8 +/- 3.9% of cryopreserved shoot tips withstood liquid nitrogen exposure and regenerated into new PLBs. CONCLUSION: These results highlighted the importance of post-rewarming osmo-conditioning for regeneration of cryopreserved shoot tips.
出版日期: 2014-03-01
卷号: 35, 期号:2, 页码:129-137
语种: 英语
收录类别: SCI
ISSN号: 0143-2044
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18223
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Lin,Liang;Xu,Qian;Ma,Junchao;Yuan,Bin;Li,Weiqi.CRYOPRESERVATION OF PROTOCORM-LIKE BODY-DERIVED SHOOT TIPS OF CALANTHE DAVIDII BY DROPLET-VITRIFICATION,CRYOLETTERS,2014,35(2):129-137
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