Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection
Zhang, Wenjing1,2; Tao, Junyan1,2; Yang, Xiaoping1,2; Yang, Zhuliang3; Zhang, Li4; Liu, Hongsheng4; Wu, Kailang1,2; Wu, Jianguo1,2; Wu,JG (reprint author),Wuhan Univ,State Key Lab Virol,Wuhan 430072,Peoples R China.; jwu@whu.edu.cn
2014-07-04
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume449Issue:3Pages:307-312
AbstractEnterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential therapeutic agents to control and treat EV71 infection. (C) 2014 Elsevier Inc. All rights reserved.
KeywordEnterovirus 71 (Ev71) Virus Infection Hand-foot-mouth Disease (Hfmd) Ganoderma Lucidum Triterpenoids (Glts) Antiviral Activity Potential Therapeutic Antiviral Agents
DOI10.1016/j.bbrc.2014.05.019
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000337781500009
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18209
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorWu,JG (reprint author),Wuhan Univ,State Key Lab Virol,Wuhan 430072,Peoples R China.; jwu@whu.edu.cn
Affiliation1.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
4.Liaoning Univ, Dept Acad Sci, Shenyang 110036, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wenjing,Tao, Junyan,Yang, Xiaoping,et al. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,449(3):307-312.
APA Zhang, Wenjing.,Tao, Junyan.,Yang, Xiaoping.,Yang, Zhuliang.,Zhang, Li.,...&jwu@whu.edu.cn.(2014).Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,449(3),307-312.
MLA Zhang, Wenjing,et al."Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 449.3(2014):307-312.
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