Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection
Zhang, Wenjing1,2; Tao, Junyan1,2; Yang, Xiaoping1,2; Yang, Zhuliang3; Zhang, Li4; Liu, Hongsheng4; Wu, Kailang1,2; Wu, Jianguo1,2; Wu,JG (reprint author),Wuhan Univ,State Key Lab Virol,Wuhan 430072,Peoples R China.; jwu@whu.edu.cn
2014-07-04
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
卷号449期号:3页码:307-312
摘要Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential therapeutic agents to control and treat EV71 infection. (C) 2014 Elsevier Inc. All rights reserved.
关键词Enterovirus 71 (Ev71) Virus Infection Hand-foot-mouth Disease (Hfmd) Ganoderma Lucidum Triterpenoids (Glts) Antiviral Activity Potential Therapeutic Antiviral Agents
资助信息Major State Basic Research Development Program (973 Program) [2012CB518900];National Natural Science Foundation of China [31230005, 81171525];National Mega Project on Major Infectious Disease Prevention [2012ZX10002006-003, 2012ZX10004-207];National Mega Project on Major Drug Development [2011ZX09401-302]
DOI10.1016/j.bbrc.2014.05.019
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000337781500009
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18209
专题中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Wu,JG (reprint author),Wuhan Univ,State Key Lab Virol,Wuhan 430072,Peoples R China.; jwu@whu.edu.cn
作者单位1.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
4.Liaoning Univ, Dept Acad Sci, Shenyang 110036, Peoples R China
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Zhang, Wenjing,Tao, Junyan,Yang, Xiaoping,et al. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,449(3):307-312.
APA Zhang, Wenjing.,Tao, Junyan.,Yang, Xiaoping.,Yang, Zhuliang.,Zhang, Li.,...&jwu@whu.edu.cn.(2014).Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,449(3),307-312.
MLA Zhang, Wenjing,et al."Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 449.3(2014):307-312.
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