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Effect of Toona microcarpa Harms Leaf Extract on the Coagulation System
Chen, Hao1,2; Jin, Min1,2; Wang, Yi-Fen3; Wang, Yong-Qing2; Meng, Ling2; Li, Rong3; Wang, Jia-Ping3; Gao, Li3; Kong, Yi1; Wei, Ji-Fu2; Kong,Y (reprint author),China Pharmaceut Univ,Sch Life Sci & Technol,Nanjing 210009,Jiangsu,Peoples R China.; yikong668@163.com; weijifu@hotmail.com
2014
发表期刊BIOMED RESEARCH INTERNATIONAL
ISSN2314-6133
页码615363
摘要Toona microcarpa Harms is a tonic, antiperiodic, antirheumatic, and antithrombotic agent in China and India and an astringent and tonic for treating diarrhea, dysentery, and other intestinal infections in Indonesia. In this study, we prepared ethyl-acetate extract from the air-dried leaves of Toona microcarpa Harms and investigated the anticoagulant activities in vitro by performing activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) assays. Antiplatelet aggregation activity of the extract was examined using adenosine diphosphate (ADP), collagen, and thrombin as agonists, and the inhibitions of factor Xa and thrombin were also investigated. Bleeding and clotting times in mice were used to determine its anticoagulant activities in vivo. It is found that Toona microcarpa Harms leaf extract (TMHE) prolonged APTT, PT, and TT clotting times in a dose-dependent manner and significantly inhibited platelet aggregation induced by thrombin, but not ADP or collagen. Clotting time and bleeding time assays showed that TMHE significantly prolonged clotting and bleeding times in vivo. In addition, at the concentration of 1 mg/mL, TMHE inhibited human thrombin activity by 73.98 +/- 2.78%. This is the first report to demonstrate that THME exhibits potent anticoagulant effects, possibly via inhibition of thrombin activity.
关键词Platelet-aggregation Thrombin Honghua
资助信息Chinese National Natural Science Foundation [31340073, 81273274, 81273375]; Jiangsu Province's Key Provincial Talents Program [RC201170]; National Major Scientific and Technological Special Project for "Significant New Drugs Development" [2011ZX09302-003-02]; Jiangsu Province Major Scientific and Technological Special Project [BM2011017]; Priority Academic Program Development of Jiangsu Higher Education Institutions
学科领域Biotechnology & Applied Microbiology ; Research & Experimental Medicine
DOI10.1155/2014/615363
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号WOS:000334845100001
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18163
专题中国西南野生生物种质资源库
通讯作者Kong,Y (reprint author),China Pharmaceut Univ,Sch Life Sci & Technol,Nanjing 210009,Jiangsu,Peoples R China.; yikong668@163.com; weijifu@hotmail.com
作者单位1.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China
2.Nanjing Med Univ, Affiliated Hosp 1, Res Div Clin Pharmacol, Nanjing 210029, Jiangsu, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Heilongtan 650201, Kunming, Peoples R China
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GB/T 7714
Chen, Hao,Jin, Min,Wang, Yi-Fen,et al. Effect of Toona microcarpa Harms Leaf Extract on the Coagulation System[J]. BIOMED RESEARCH INTERNATIONAL,2014:615363.
APA Chen, Hao.,Jin, Min.,Wang, Yi-Fen.,Wang, Yong-Qing.,Meng, Ling.,...&weijifu@hotmail.com.(2014).Effect of Toona microcarpa Harms Leaf Extract on the Coagulation System.BIOMED RESEARCH INTERNATIONAL,615363.
MLA Chen, Hao,et al."Effect of Toona microcarpa Harms Leaf Extract on the Coagulation System".BIOMED RESEARCH INTERNATIONAL (2014):615363.
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