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Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus
Lu, Zhonglei1; Kong, Xiangxiang2; Lu, Zhaoming1; Xiao, Meixiang1; Chen, Meiyuan3; Zhu, Liang4,5; Shen, Yuemao1; Hu, Xiangyang2; Song, Siyang1
Corresponding AuthorHu, XY (reprint author), Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming, Yunnan, Peoples R China. ; huxiangyang@mail.kib.ac.cn ; sysong@xmu.edu.cn
2014-03-10
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:3Pages:e91298
AbstractMost mushrooms are thermo-sensitive to temperatures over 23 degrees C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermosensitive. Here, we compared their responses at proteomic level to heat treatment at 33 degrees C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e. g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.
KeywordPrecursor P-aminobenzoate Salicylic-acid Temperature Stress Oxidative Stress Gene-expression Protective Role Abiotic Stress Glucose Ester Abscisic-acid Salt Stress
Subject AreaMultidisciplinary Sciences
DOI10.1371/journal.pone.0091298
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000332839300107
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18072
Collection中国科学院青藏高原研究所昆明部
Affiliation1.Xiamen Univ, Sch Life Sci, State Key Lab Stress Cell Biol, Xiamen, Fujian, Peoples R China
2.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
3.Fujian Acad Agr Sci, Edible Fungi Inst, Fuzhou, Fujian, Peoples R China
4.Albert Einstein Coll Med, Dept Dev & Mol Biol, Bronx, NY 10467 USA
5.Albert Einstein Coll Med, Bronx, NY 10467 USA
Recommended Citation
GB/T 7714
Lu, Zhonglei,Kong, Xiangxiang,Lu, Zhaoming,et al. Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus[J]. PLOS ONE,2014,9(3):e91298.
APA Lu, Zhonglei.,Kong, Xiangxiang.,Lu, Zhaoming.,Xiao, Meixiang.,Chen, Meiyuan.,...&Song, Siyang.(2014).Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus.PLOS ONE,9(3),e91298.
MLA Lu, Zhonglei,et al."Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus".PLOS ONE 9.3(2014):e91298.
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