Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea
Chen, Shaoxing1,2; Wang, Chuanming2; Xu, Jian-Ping3; Yang, Zhu L.1
Corresponding AuthorYang,ZL (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Yunnan,Peoples R China. ; haloarchaea1979@gmail.com ; wcm_biology2@126.com ; fungi@mail.kib.ac.cn
2014-03-01
Source PublicationEXTREMOPHILES
ISSN1431-0651
Volume18Issue:2Pages:195-206
AbstractA novel haloarchaeal plasmid, pHRDV1 (13,053 bp), was isolated from the haloarchaeal isolate Halorubrum sp. T3. Molecular and bioinformatics analyses showed that this element is a double-stranded circular DNA molecule containing two putative transcripts with opposite directions. The amino acid sequences of six of the nineteen predicted open reading frames were similar to those found in haloarchaeal pleomorphic viruses, such as Halorubrum pleomorphic virus 3 and Halogeometricum pleomorphic virus 1. There was also a strong conservation in gene order between the plasmid and these viruses. All three conserved viral proteins (VPs), which are characteristic of haloarchaeal pleomorphic viruses VP3, VP4 and VP8, were found in pHRDV1. Furthermore, a typical repressor-operator system similar to haloarchaeal myovirus phi Ch1, was found on the genome of pHRDV1. However, no viral particles were detected in the supernatants of Halorubrum sp. T3, either in the presence or absence of mitomycin C. These results imply that plasmid pHRDV1 is a distinctive virus-like mobile genetic element that harbors some unique properties that make it different from all of the known haloarchaeal plasmids or viruses.
KeywordHalorubrum Hypersaline Environment Mobile Genetic Elements Horizontal Gene Transfer
Subject AreaBiochemistry & Molecular Biology ; Microbiology
DOI10.1007/s00792-013-0599-4
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Microbiology
WOS IDWOS:000331970700001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/17951
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China
2.Honghe Univ, Univ Key Lab Crop High Qual & High Effect Cultiva, Mengzi, Peoples R China
3.McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada
Recommended Citation
GB/T 7714
Chen, Shaoxing,Wang, Chuanming,Xu, Jian-Ping,et al. Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea[J]. EXTREMOPHILES,2014,18(2):195-206.
APA Chen, Shaoxing,Wang, Chuanming,Xu, Jian-Ping,&Yang, Zhu L..(2014).Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea.EXTREMOPHILES,18(2),195-206.
MLA Chen, Shaoxing,et al."Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea".EXTREMOPHILES 18.2(2014):195-206.
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