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题名: Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea
作者: Chen, Shaoxing1, 2; Wang, Chuanming2; Xu, Jian-Ping3; null(杨祝良)1
刊名: EXTREMOPHILES
关键词: Halorubrum ; Hypersaline environment ; Mobile genetic elements ; Horizontal gene transfer
英文摘要: A novel haloarchaeal plasmid, pHRDV1 (13,053 bp), was isolated from the haloarchaeal isolate Halorubrum sp. T3. Molecular and bioinformatics analyses showed that this element is a double-stranded circular DNA molecule containing two putative transcripts with opposite directions. The amino acid sequences of six of the nineteen predicted open reading frames were similar to those found in haloarchaeal pleomorphic viruses, such as Halorubrum pleomorphic virus 3 and Halogeometricum pleomorphic virus 1. There was also a strong conservation in gene order between the plasmid and these viruses. All three conserved viral proteins (VPs), which are characteristic of haloarchaeal pleomorphic viruses VP3, VP4 and VP8, were found in pHRDV1. Furthermore, a typical repressor-operator system similar to haloarchaeal myovirus phi Ch1, was found on the genome of pHRDV1. However, no viral particles were detected in the supernatants of Halorubrum sp. T3, either in the presence or absence of mitomycin C. These results imply that plasmid pHRDV1 is a distinctive virus-like mobile genetic element that harbors some unique properties that make it different from all of the known haloarchaeal plasmids or viruses.
出版日期: 2014-03-01
卷号: 18, 期号:2, 页码:195-206
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology ; Microbiology
ISSN号: 1431-0651
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/17951
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China
2.Honghe Univ, Univ Key Lab Crop High Qual & High Effect Cultiva, Mengzi, Peoples R China
3.McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada

Recommended Citation:
Chen,Shaoxing;Wang,Chuanming;Xu,JianPing;Yang,Zhu L.*.Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea,EXTREMOPHILES,2014,18(2):195-206
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