Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province | |
Zhang, Dequan1; Yang, Jing2![]() | |
通讯作者 | Jiang,B (reprint author),Dali Univ,Coll Pharm & Chem,Dali 671000,Yunnan,Peoples R China.,northjiang@yahoo.com |
2013-10-01 | |
发表期刊 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
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ISSN | 0305-1978 |
卷号 | 50页码:448-451 |
摘要 | Astragalus camptodontus is a traditional medicinal plant in Yunnan province, and it is used as one of substitutes for the Chinese medicine Astragali radix, namely "Huangqi". Twenty-three microsatellite loci were developed from its nuclear genome using a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Two wild populations collected from Northwest Yunnan were used to assess polymorphism of each locus. The number of alleles per locus ranged from 2 to 5 with a mean of 4.2. Values for Ho and HE ranged from 0.042 to 0.875 and from 0.254 to 0.793, with average of 0.403 and 0.616, respectively. These polymorphic loci will facilitate further studies on genetic diversity, population structure and gene flow of A. camptodontus and its allied species. (C) 2013 Elsevier Ltd. All rights reserved. |
关键词 | Astragalus Camptodontus Medicinal Plant Microsatellite Fiasco Genetic Diversity |
学科领域 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000325597600065 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/17446 |
专题 | 中国西南野生生物种质资源库 |
作者单位 | 1.Dali Univ, Coll Pharm & Chem, Dali 671000, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Dequan,Yang, Jing,Jiang, Bei. Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2013,50:448-451. |
APA | Zhang, Dequan,Yang, Jing,&Jiang, Bei.(2013).Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,50,448-451. |
MLA | Zhang, Dequan,et al."Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 50(2013):448-451. |
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