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题名: Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province
作者: Zhang, Dequan1; Yang, Jing2; Jiang, Bei1
刊名: BIOCHEMICAL SYSTEMATICS AND ECOLOGY
关键词: Astragalus camptodontus ; Medicinal plant ; Microsatellite ; FIASCO ; Genetic diversity
英文摘要: Astragalus camptodontus is a traditional medicinal plant in Yunnan province, and it is used as one of substitutes for the Chinese medicine Astragali radix, namely "Huangqi". Twenty-three microsatellite loci were developed from its nuclear genome using a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Two wild populations collected from Northwest Yunnan were used to assess polymorphism of each locus. The number of alleles per locus ranged from 2 to 5 with a mean of 4.2. Values for Ho and HE ranged from 0.042 to 0.875 and from 0.254 to 0.793, with average of 0.403 and 0.616, respectively. These polymorphic loci will facilitate further studies on genetic diversity, population structure and gene flow of A. camptodontus and its allied species. (C) 2013 Elsevier Ltd. All rights reserved.
出版日期: 2013-10-01
卷号: 50, 页码:448-451
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
ISSN号: 0305-1978
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/17446
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Dali Univ, Coll Pharm & Chem, Dali 671000, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Yunnan, Peoples R China

Recommended Citation:
Zhang,Dequan;Yang,Jing;Jiang,Bei.Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province,BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2013,50():448-451
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