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Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province
Zhang, Dequan1; Yang, Jing2; Jiang, Bei1
2013-10-01
发表期刊BIOCHEMICAL SYSTEMATICS AND ECOLOGY
ISSN0305-1978
卷号50页码:448-451
摘要Astragalus camptodontus is a traditional medicinal plant in Yunnan province, and it is used as one of substitutes for the Chinese medicine Astragali radix, namely "Huangqi". Twenty-three microsatellite loci were developed from its nuclear genome using a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Two wild populations collected from Northwest Yunnan were used to assess polymorphism of each locus. The number of alleles per locus ranged from 2 to 5 with a mean of 4.2. Values for Ho and HE ranged from 0.042 to 0.875 and from 0.254 to 0.793, with average of 0.403 and 0.616, respectively. These polymorphic loci will facilitate further studies on genetic diversity, population structure and gene flow of A. camptodontus and its allied species. (C) 2013 Elsevier Ltd. All rights reserved.
关键词Astragalus Camptodontus Medicinal Plant Microsatellite Fiasco Genetic Diversity
资助信息National Natural Science Foundation of China [31170313];Research Fund for the Large-scale Scientific Facilities of the Chinese Academy of Sciences [2009-LSF-GBOWS-01]
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17446
专题中国西南野生生物种质资源库
作者单位1.Dali Univ, Coll Pharm & Chem, Dali 671000, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Yunnan, Peoples R China
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Zhang, Dequan,Yang, Jing,Jiang, Bei. Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2013,50:448-451.
APA Zhang, Dequan,Yang, Jing,&Jiang, Bei.(2013).Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,50,448-451.
MLA Zhang, Dequan,et al."Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 50(2013):448-451.
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