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Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death
Chen, Hongying1,2; Osuna, Daniel3; Colville, Louise2; Lorenzo, Oscar3; Graeber, Kai4,5; Kuester, Helge5; Leubner-Metzger, Gerhard4; Kranner, Ilse2
Corresponding AuthorColville,L (reprint author),Kew,Royal Bot Gardens,Seed Conservat Dept,Ardingly,W Sussex,England.,l.colville@kew.org
2013-10-29
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:10
AbstractUnderstanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential for regulation at the transcriptional level has not been fully investigated. Here, we studied the relationship between seed viability, gene expression and glutathione redox status during artificial ageing of pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant glutathione. Partial degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50 degrees C, and transcriptome profiling showed that the expression of genes associated with programmed cell death, oxidative stress and protein ubiquitination were altered prior to any sign of viability loss. After 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions, as indicated by a shift of the glutathione redox state towards more positive values (>-190 mV). The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process, which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.
KeywordProtein S-glutathionylation Signal-regulating Kinase-1 Arabidopsis-thaliana Bax Inhibitor-1 Differential Expression Lipid-peroxidation Induced Apoptosis Messenger-rna Nicotiana-benthamiana Microarray Analysis
Subject AreaScience & Technology - Other Topics
DOI10.1371/journal.pone.0078471
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000326270700090
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/17439
Collection中国西南野生生物种质资源库
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
2.Kew, Royal Bot Gardens, Seed Conservat Dept, Ardingly, W Sussex, England
3.Univ Salamanca, Fac Biol, Ctr Hispano Luso Invest Agr CIALE, Dept Fisiol Vegetal, E-37008 Salamanca, Spain
4.Univ Freiburg, Fac Biol, Inst Biol 2, D-79106 Freiburg, Germany
5.Leibniz Univ Hannover, Inst Plant Genet, Unit Plant Genom 4, D-30167 Hannover, Germany
Recommended Citation
GB/T 7714
Chen, Hongying,Osuna, Daniel,Colville, Louise,et al. Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death[J]. PLOS ONE,2013,8(10).
APA Chen, Hongying.,Osuna, Daniel.,Colville, Louise.,Lorenzo, Oscar.,Graeber, Kai.,...&Kranner, Ilse.(2013).Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death.PLOS ONE,8(10).
MLA Chen, Hongying,et al."Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death".PLOS ONE 8.10(2013).
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