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Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death
Chen, Hongying1,2; Osuna, Daniel3; Colville, Louise2; Lorenzo, Oscar3; Graeber, Kai4,5; Kuester, Helge5; Leubner-Metzger, Gerhard4; Kranner, Ilse2
2013-10-29
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:10
摘要Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential for regulation at the transcriptional level has not been fully investigated. Here, we studied the relationship between seed viability, gene expression and glutathione redox status during artificial ageing of pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant glutathione. Partial degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50 degrees C, and transcriptome profiling showed that the expression of genes associated with programmed cell death, oxidative stress and protein ubiquitination were altered prior to any sign of viability loss. After 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions, as indicated by a shift of the glutathione redox state towards more positive values (>-190 mV). The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process, which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.
关键词Protein S-glutathionylation Signal-regulating Kinase-1 Arabidopsis-thaliana Bax Inhibitor-1 Differential Expression Lipid-peroxidation Induced Apoptosis Messenger-rna Nicotiana-benthamiana Microarray Analysis
资助信息Spanish Ministerio de Educacion y Ciencia;Junta de Castilla y Leon [BIO2011-26940, CSD2007-00057, SA048A10-2];German Research Foundation [DFGLe720/ 7];Chinese Academy of Sciences [KSCX2-EW-J-24, Y3221411W1];Millennium Commission;Wellcome Trust;Orange Plc.;Defra
学科领域Science & Technology - Other Topics
DOI10.1371/journal.pone.0078471
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000326270700090
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17439
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
2.Kew, Royal Bot Gardens, Seed Conservat Dept, Ardingly, W Sussex, England
3.Univ Salamanca, Fac Biol, Ctr Hispano Luso Invest Agr CIALE, Dept Fisiol Vegetal, E-37008 Salamanca, Spain
4.Univ Freiburg, Fac Biol, Inst Biol 2, D-79106 Freiburg, Germany
5.Leibniz Univ Hannover, Inst Plant Genet, Unit Plant Genom 4, D-30167 Hannover, Germany
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Chen, Hongying,Osuna, Daniel,Colville, Louise,et al. Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death[J]. PLOS ONE,2013,8(10).
APA Chen, Hongying.,Osuna, Daniel.,Colville, Louise.,Lorenzo, Oscar.,Graeber, Kai.,...&Kranner, Ilse.(2013).Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death.PLOS ONE,8(10).
MLA Chen, Hongying,et al."Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death".PLOS ONE 8.10(2013).
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