KIB OpenIR  > 中国西南野生生物种质资源库
Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death
Chen, Hongying1,2; Osuna, Daniel3; Colville, Louise2; Lorenzo, Oscar3; Graeber, Kai4,5; Kuester, Helge5; Leubner-Metzger, Gerhard4; Kranner, Ilse2
通讯作者Colville,L (reprint author),Kew,Royal Bot Gardens,Seed Conservat Dept,Ardingly,W Sussex,England.,l.colville@kew.org
2013-10-29
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:10
摘要Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential for regulation at the transcriptional level has not been fully investigated. Here, we studied the relationship between seed viability, gene expression and glutathione redox status during artificial ageing of pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant glutathione. Partial degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50 degrees C, and transcriptome profiling showed that the expression of genes associated with programmed cell death, oxidative stress and protein ubiquitination were altered prior to any sign of viability loss. After 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions, as indicated by a shift of the glutathione redox state towards more positive values (>-190 mV). The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process, which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.
关键词Protein S-glutathionylation Signal-regulating Kinase-1 Arabidopsis-thaliana Bax Inhibitor-1 Differential Expression Lipid-peroxidation Induced Apoptosis Messenger-rna Nicotiana-benthamiana Microarray Analysis
学科领域Science & Technology - Other Topics
DOI10.1371/journal.pone.0078471
收录类别SCI
语种英语
WOS记录号WOS:000326270700090
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17439
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
2.Kew, Royal Bot Gardens, Seed Conservat Dept, Ardingly, W Sussex, England
3.Univ Salamanca, Fac Biol, Ctr Hispano Luso Invest Agr CIALE, Dept Fisiol Vegetal, E-37008 Salamanca, Spain
4.Univ Freiburg, Fac Biol, Inst Biol 2, D-79106 Freiburg, Germany
5.Leibniz Univ Hannover, Inst Plant Genet, Unit Plant Genom 4, D-30167 Hannover, Germany
推荐引用方式
GB/T 7714
Chen, Hongying,Osuna, Daniel,Colville, Louise,et al. Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death[J]. PLOS ONE,2013,8(10).
APA Chen, Hongying.,Osuna, Daniel.,Colville, Louise.,Lorenzo, Oscar.,Graeber, Kai.,...&Kranner, Ilse.(2013).Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death.PLOS ONE,8(10).
MLA Chen, Hongying,et al."Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death".PLOS ONE 8.10(2013).
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Chen-2013-Transcript(2073KB) 开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Hongying]的文章
[Osuna, Daniel]的文章
[Colville, Louise]的文章
百度学术
百度学术中相似的文章
[Chen, Hongying]的文章
[Osuna, Daniel]的文章
[Colville, Louise]的文章
必应学术
必应学术中相似的文章
[Chen, Hongying]的文章
[Osuna, Daniel]的文章
[Colville, Louise]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Chen-2013-Transcriptome-Wide M.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。