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题名: 白菊木和栌菊木的保护遗传学及亲缘关系研究 
作者: 赵玉娟
学位类别: 博士
答辩日期: 2011
授予单位: 中国科学院研究生院
导师: 龚洵
关键词: 白菊木 ; 栌菊木 ; 保护遗传学 ; 遗传多样性 ; 居群遗传结构 ; 亲缘关系
学科分类: Conservation genetics of L. decora: The nucleotide sequences from three chloroplast DNA (cpDNA) regions (the rpl16 intron, trnQ-5’rps16 intergenic spacer and rpl32-trnL intergenic spacer) and two nuclear gene (GAPDH and GBSSI) were selected to characterize the genetic structure and phylogeography of this species. Eleven populations were sampled across the distribution range of L. decora in China. Low levels of cpDNA genetic diversity were found in this species and within populations, with the identification of 2 haplotypes in a total of 2745 bp (cpDNA: π = 0.00102, Hd = 0.468, HT = 0.480), while the levels of genetic diversity revealed by the nuclear gene GAPDH and GBSSI (GAPDH: π = 0.00237, Hd = 0.773, HT = 0.807; GBSSI: π = 0.00167, Hd = 0.751, HT = 0.790) were relatively high, indicating that random losses of genetic polymorphisms from populations may have occurred recently. Relatively high genetic differentiation for both markers (cpDNA: GST = 0.899, FST = 0.893; GAPDH: GST = 0.309, FST = 0.432; GBSSI: GST = 0.274, FST = 0.263) were detected at the wide-species level. Two cpDNA haplotypes, twelve GAPDH haplotypes and ten GBSSI haplotypes were recovered, respectively. Almost every population except the population SP was fixed for one cpDNA haplotype, causing no variation within the populations. Several nuclear haplotypes were distributed widely in most populations. These results may be mainly explained by long-term fragmentation and the limited gene flow caused by geographic isolation among populations. The mismatch distribution analyses and neutrality tests provided no evidence for a recent population expansion in L. decora. The time of all GAPDH haplotypes coalesced to the most recent common ancestor was dated to between 0.46 (95% CI 0.14-0.89)-0.65 (95% CI 0.18-1.22)mya, which predates the LGM. In situ shrinking or extinction might have occurred to L. decora during the glacial ages and L. decora populations did not retreat to common refugia or migrated backwards. Based on the genetic diversity and uniqueness of the populations, conservation strategies are discussed for this endangered species.2.
中文摘要: 白菊木(Leucomeris decora Kurz)和栌菊木(Nouelia insignis Franch.)同属菊科(Asteraceae)帚菊木族(Mutisieae)植物类群,都为我国珍稀濒危植物。本研究基于叶绿体DNA(cpDNA)非编码区序列以及核基因(nDNA)序列的变异,对白菊木和栌菊木的遗传多样性、遗传结构和系统发育进行了研究,并探讨了其相应的保护策略。主要的研究结果如下:1.
语种: 中文
内容类型: 学位论文
URI标识: http://ir.kib.ac.cn/handle/151853/16999
Appears in Collections:昆明植物所硕博研究生毕业学位论文_学位论文

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Recommended Citation:
赵玉娟.白菊木和栌菊木的保护遗传学及亲缘关系研究 .[博士 学位论文 ].中国科学院研究生院 .2011
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