KIB OpenIR  > 中国西南野生生物种质资源库
The expression and phylogenetic analysis of four AP3-like paralogs in the stamens, carpels, and single-whorl perianth of the paleoherb Asarum caudigerum
Zhao, Yin-He1,2; Larson-Rabin, Zachary3; Li, De-Zhu3; Wang, Guo-Ying4; Peng, Seng1,2; Li, Cheng-Yun1,2
2013-08-01
发表期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
卷号40期号:8页码:4691-4699
摘要The paleoherb species Asarum caudigerum (Aristolochiaceae) is important for research into the origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, four MADS-box-containing transcripts were isolated from A. caudigerum by rapid amplification of cDNA ends (RACE). Sequence comparisons and phylogenetic analyses indicated that they possess high homology to AP3 subfamily genes, which have been shown previously to be involved in petal and stamen development in eudicots. Reverse-transcription quantitative PCR (RT-qPCR) and in situ hybridization analyses showed AcAP3-A expression mainly in the second whorl (stamens) and AcAP3-B expression in whorls 1 and 3 (perianth and carpels). Compared with eudicot AP3 homologs, premature translation termination codons were caused by an insertion in the K1 domain of AcAP3-C, and by a deletion in the 7th exon of AcAP3-D. Sequence analyses suggested that the A. caudigerum AP3 lineage had undergone gene duplication and subfunctionalization, diverging in expression patterns during perianth, stamen, and carpel development. Based on comparative genomic and phylogenetic analyses, we concluded that subfunctionalization has likely contributed to the persistence of two functional AP3 paralogs, that two other copies may have become pseudogenes, and that these AP3 duplication and subfunctionalization events may have contributed to the evolution of the unusual floral morphology of A. caudigerum.
关键词Asarum Caudigerum Ap3-like Gene Duplication Pseudogene Neofunctionalization Subfunctionalization
资助信息National Natural Science Foundation of China [NSFC30860130, NSFC31060166]; Young Academic and Technical Leader Raising Foundation of Yunnan Province [2012HB018]; Large-scale Scientific Facilities of the Chinese Academy of Sciences [2009-LSFGBOWS-01]; Chinese Academy of Sciences Young International Scientist Fellowship; National Basic Research Program of China [2012CB722900]
学科领域Biochemistry & Molecular Biology
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000322392300003
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16873
专题中国西南野生生物种质资源库
作者单位1.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Peoples R China
2.Yunnan Agr Univ, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650204, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Yin-He,Larson-Rabin, Zachary,Li, De-Zhu,et al. The expression and phylogenetic analysis of four AP3-like paralogs in the stamens, carpels, and single-whorl perianth of the paleoherb Asarum caudigerum[J]. MOLECULAR BIOLOGY REPORTS,2013,40(8):4691-4699.
APA Zhao, Yin-He,Larson-Rabin, Zachary,Li, De-Zhu,Wang, Guo-Ying,Peng, Seng,&Li, Cheng-Yun.(2013).The expression and phylogenetic analysis of four AP3-like paralogs in the stamens, carpels, and single-whorl perianth of the paleoherb Asarum caudigerum.MOLECULAR BIOLOGY REPORTS,40(8),4691-4699.
MLA Zhao, Yin-He,et al."The expression and phylogenetic analysis of four AP3-like paralogs in the stamens, carpels, and single-whorl perianth of the paleoherb Asarum caudigerum".MOLECULAR BIOLOGY REPORTS 40.8(2013):4691-4699.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Zhao-2013-The expres(791KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Yin-He]的文章
[Larson-Rabin, Zachary]的文章
[Li, De-Zhu]的文章
百度学术
百度学术中相似的文章
[Zhao, Yin-He]的文章
[Larson-Rabin, Zachary]的文章
[Li, De-Zhu]的文章
必应学术
必应学术中相似的文章
[Zhao, Yin-He]的文章
[Larson-Rabin, Zachary]的文章
[Li, De-Zhu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。