Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation

	Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation
	Jia, Yanxia 1,3; Tao, Faqing 2; Li, Weiqi1,2
通讯作者	Li, WQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China. ; weiqili@mail.kib.ac.cn
	2013-06-07
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:6 页码:e65687
摘要	Senescence is the last phase of the plant life cycle and has an important role in plant development. Degradation of membrane lipids is an essential process during leaf senescence. Several studies have reported fundamental changes in membrane lipids and phospholipase D (PLD) activity as leaves senesce. Suppression of phospholipase D alpha 1 (PLD alpha 1) retards abscisic acid (ABA)-promoted senescence. However, given the absence of studies that have profiled changes in the compositions of membrane lipid molecules during leaf senescence, there is no direct evidence that PLD affects lipid composition during the process. Here, we show that application of n-butanol, an inhibitor of PLD, and N-Acylethanolamine (NAE) 12:0, a specific inhibitor of PLD alpha 1, retarded ABA-promoted senescence to different extents. Furthermore, phospholipase D delta (PLD delta) was induced in leaves treated with ABA, and suppression of PLD delta retarded ABA-promoted senescence in Arabidopsis. Lipid profiling revealed that detachment-induced senescence had different effects on plastidic and extraplastidic lipids. The accelerated degradation of plastidic lipids during ABA-induced senescence in wild-type plants was attenuated in PLD delta-knockout (PLD delta-KO) plants. Dramatic increases in phosphatidic acid (PA) and decreases in phosphatidylcholine (PC) during ABA-induced senescence were also suppressed in PLD delta-KO plants. Our results suggest that PLD delta-mediated hydrolysis of PC to PA plays a positive role in ABA-promoted senescence. The attenuation of PA formation resulting from suppression of PLD delta blocks the degradation of membrane lipids, which retards ABA-promoted senescence.
关键词	Phosphatidic-acid Plasma-membrane D-alpha Microsomal-membranes Carnation Flowers Tobacco Plants d Activation Pld-delta Thaliana Metabolism
学科领域	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000321094800069
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16869
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yanxia,Tao, Faqing,Li, Weiqi. Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation[J]. PLOS ONE,2013,8(6):e65687.
APA	Jia, Yanxia,Tao, Faqing,&Li, Weiqi.(2013).Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation.PLOS ONE,8(6),e65687.
MLA	Jia, Yanxia,et al."Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation".PLOS ONE 8.6(2013):e65687.

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