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Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation
Jia, Yanxia1,3; Tao, Faqing2; Li, Weiqi1,2
Corresponding AuthorLi, WQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China. ; weiqili@mail.kib.ac.cn
2013-06-07
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:6Pages:e65687
AbstractSenescence is the last phase of the plant life cycle and has an important role in plant development. Degradation of membrane lipids is an essential process during leaf senescence. Several studies have reported fundamental changes in membrane lipids and phospholipase D (PLD) activity as leaves senesce. Suppression of phospholipase D alpha 1 (PLD alpha 1) retards abscisic acid (ABA)-promoted senescence. However, given the absence of studies that have profiled changes in the compositions of membrane lipid molecules during leaf senescence, there is no direct evidence that PLD affects lipid composition during the process. Here, we show that application of n-butanol, an inhibitor of PLD, and N-Acylethanolamine (NAE) 12:0, a specific inhibitor of PLD alpha 1, retarded ABA-promoted senescence to different extents. Furthermore, phospholipase D delta (PLD delta) was induced in leaves treated with ABA, and suppression of PLD delta retarded ABA-promoted senescence in Arabidopsis. Lipid profiling revealed that detachment-induced senescence had different effects on plastidic and extraplastidic lipids. The accelerated degradation of plastidic lipids during ABA-induced senescence in wild-type plants was attenuated in PLD delta-knockout (PLD delta-KO) plants. Dramatic increases in phosphatidic acid (PA) and decreases in phosphatidylcholine (PC) during ABA-induced senescence were also suppressed in PLD delta-KO plants. Our results suggest that PLD delta-mediated hydrolysis of PC to PA plays a positive role in ABA-promoted senescence. The attenuation of PA formation resulting from suppression of PLD delta blocks the degradation of membrane lipids, which retards ABA-promoted senescence.
KeywordPhosphatidic-acid Plasma-membrane D-alpha Microsomal-membranes Carnation Flowers Tobacco Plants d Activation Pld-delta Thaliana Metabolism
Subject AreaMultidisciplinary Sciences
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000321094800069
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/16869
Collection中国西南野生生物种质资源库
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Jia, Yanxia,Tao, Faqing,Li, Weiqi. Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation[J]. PLOS ONE,2013,8(6):e65687.
APA Jia, Yanxia,Tao, Faqing,&Li, Weiqi.(2013).Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation.PLOS ONE,8(6),e65687.
MLA Jia, Yanxia,et al."Lipid Profiling Demonstrates That Suppressing Arabidopsis Phospholipase D delta Retards ABA-Promoted Leaf Senescence by Attenuating Lipid Degradation".PLOS ONE 8.6(2013):e65687.
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