MS-desi, a desiccation-related protein in the floral nectar of the evergreen velvet bean (Mucuna sempervirens Hemsl): molecular identification and characterization
Zha, Hong-Guang1; Liu, Tao2; Zhou, Jing-Jiang3; Sun, Hang1
2013-07-01
发表期刊PLANTA
ISSN0032-0935
卷号238期号:1页码:77-89
摘要Plant desiccation-related proteins (DRPs) were first identified as pcC13-62 from the resurrection plant Craterostigma plantagineum and it has been suggested they are involved in plant desiccation tolerance. We identified and beancharacterized a plant DRP, which we called MS-desi, in the floral nectar of a subtropical species, Mucuna sempervirens (MS). MS-desi is a major nectar protein (nectarin) of the bean plant and expresses exclusively in the stylopodium, where the nectary is located. The full-length MS-desi gene encodes for a protein of 306 amino acids with a molecular mass of 33,248 Da, and possesses a ferritin-like domain and a signal peptide of 30 amino acids. Structural and phylogenetic analysis demonstrated MS-desi has high similarity to members of the plant DRPs, including pcC 13-62 protein. MS-desi has a similar hydropathy profile to that of pcC13-62 with a grand average of hydropathy index of 0.130 for MS-desi and 0.106 for pcC13-62 protein, which is very different from those of dehydrins and late embryogenesis abundant proteins. The protein's secondary structures, both predicted from the amino acid sequence and directly analysed by far UV circular dichroism, showed that MS-desi is mainly composed of alpha helices and is relatively temperature dependent. The structure change is reversible within a wide range of temperatures. Purified MS-desi and raw MS floral nectar showed dose-dependent citrate synthase inhibition activity, but insensitivity to lactate dehydrogenase, suggesting that, unlike dehydrins, it does not act as a chaperone. The overall results constitute, to our knowledge, the first study on a desiccation-related protein in plant floral nectar.
关键词Citrate Synthase Inhibition Desiccation-related Protein Mucuna Nectarin Pcc13-62 Like
资助信息West Light Foundation of The Chinese Academy of Sciences; National Science Foundation of China [31170216]; National Natural Science Foundation; Yunnan Natural Science Foundation [U136601]; Biotechnology and Biological Sciences Research Council of the UK
学科领域Plant Sciences
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000320961900006
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16805
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China
3.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England
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Zha, Hong-Guang,Liu, Tao,Zhou, Jing-Jiang,et al. MS-desi, a desiccation-related protein in the floral nectar of the evergreen velvet bean (Mucuna sempervirens Hemsl): molecular identification and characterization[J]. PLANTA,2013,238(1):77-89.
APA Zha, Hong-Guang,Liu, Tao,Zhou, Jing-Jiang,&Sun, Hang.(2013).MS-desi, a desiccation-related protein in the floral nectar of the evergreen velvet bean (Mucuna sempervirens Hemsl): molecular identification and characterization.PLANTA,238(1),77-89.
MLA Zha, Hong-Guang,et al."MS-desi, a desiccation-related protein in the floral nectar of the evergreen velvet bean (Mucuna sempervirens Hemsl): molecular identification and characterization".PLANTA 238.1(2013):77-89.
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