Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis

doi:10.1371/journal.pone.0053019

	Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis
	Zhao, Yin-He 1,2,3; Larson-Rabin, Zachary 1; Wang, Guo-Ying 4; Moeller, Michael 5; Li, Cheng-Yun 2,3; Zhang, Jin-Peng 4; Li, Hong-Tao1 ; Li, De-Zhu1
通讯作者	Li, DZ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China. ; dzl@mail.kib.ac.cn
	2013-01-30
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:1 页码:e53019
摘要	Saururus chinensis is a core member of Saururaceae, a perianthless (lacking petals or sepals) family. Due to its basal phylogenetic position and unusual floral composition, study of this plant family is important for understanding the origin and evolution of perianthless flowers and petaloid bracts among angiosperm species. To isolate genes involved in S. chinensis flower development, subtracted floral cDNA libraries were constructed by using suppression subtractive hybridization (SSH) on transcripts isolated from developing inflorescences and seedling leaves. The subtracted cDNA libraries contained a total of 1,141 ESTs and were used to create cDNA microarrays to analyze transcript profiles of developing inflorescence tissues. Subsequently, qRT-PCR analyses of eight MADS-box transcription factors and in situ hybridizations of two B-class MADS-box transcription factors were performed to verify and extend the cDNA microarray results. Finally, putative phylogenetic relationships within the B-class MADS-box gene family were determined using the discovered S. chinensis B-class genes to compare K-domain sequences with B genes from other basal angiosperms. Two hundred seventy-seven of the 1,141 genes were found to be expressed differentially between S. chinensis inflorescence tissues and seedling leaves, 176 of which were grouped into at least one functional category, including transcription (14.75%), energy (12.59%), metabolism (9.12%), protein-related function (8.99%), and cellular transport (5.76%). qRT-PCR and in situ hybridization of selected MADS-box genes supported our microarray data. Phylogenetic analysis indicated that a total of six B-class MADS-box genes were isolated from S. chinensis. The differential regulation of S. chinensis B-class MADSbox transcription factors likely plays a role during the development of subtending bracts and perianthless flowers. This study contributes to our understanding of inflorescence development in Saururus, and represents an initial step toward understanding the formation of petaloid bracts in this species.
关键词	Floral Organ Identity Mads-box Genes Basal Angiosperms Homeotic Genes Core Eudicots Arabidopsis Expression Evolution Apetala3 Pistillata
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0053019
收录类别	SCI
语种	英语
WOS记录号	WOS:000315563800017
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16625
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China 2.Yunnan Agr Univ, Coll Agron, Kunming, Peoples R China 3.Yunnan Agr Univ, Key Lab Plant Pathol Yunnan Prov, Kunming, Peoples R China 4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China 5.Royal Bot Garden Edinburgh, Edinburgh, Midlothian, Scotland
推荐引用方式 GB/T 7714	Zhao, Yin-He,Larson-Rabin, Zachary,Wang, Guo-Ying,et al. Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis[J]. PLOS ONE,2013,8(1):e53019.
APA	Zhao, Yin-He.,Larson-Rabin, Zachary.,Wang, Guo-Ying.,Moeller, Michael.,Li, Cheng-Yun.,...&Li, De-Zhu.(2013).Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis.PLOS ONE,8(1),e53019.
MLA	Zhao, Yin-He,et al."Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis".PLOS ONE 8.1(2013):e53019.

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