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Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis
Zhao, Yin-He1,2,3; Larson-Rabin, Zachary1; Wang, Guo-Ying4; Moeller, Michael5; Li, Cheng-Yun2,3; Zhang, Jin-Peng4; Li, Hong-Tao1; Li, De-Zhu1
2013-01-30
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:1页码:e53019
摘要Saururus chinensis is a core member of Saururaceae, a perianthless (lacking petals or sepals) family. Due to its basal phylogenetic position and unusual floral composition, study of this plant family is important for understanding the origin and evolution of perianthless flowers and petaloid bracts among angiosperm species. To isolate genes involved in S. chinensis flower development, subtracted floral cDNA libraries were constructed by using suppression subtractive hybridization (SSH) on transcripts isolated from developing inflorescences and seedling leaves. The subtracted cDNA libraries contained a total of 1,141 ESTs and were used to create cDNA microarrays to analyze transcript profiles of developing inflorescence tissues. Subsequently, qRT-PCR analyses of eight MADS-box transcription factors and in situ hybridizations of two B-class MADS-box transcription factors were performed to verify and extend the cDNA microarray results. Finally, putative phylogenetic relationships within the B-class MADS-box gene family were determined using the discovered S. chinensis B-class genes to compare K-domain sequences with B genes from other basal angiosperms. Two hundred seventy-seven of the 1,141 genes were found to be expressed differentially between S. chinensis inflorescence tissues and seedling leaves, 176 of which were grouped into at least one functional category, including transcription (14.75%), energy (12.59%), metabolism (9.12%), protein-related function (8.99%), and cellular transport (5.76%). qRT-PCR and in situ hybridization of selected MADS-box genes supported our microarray data. Phylogenetic analysis indicated that a total of six B-class MADS-box genes were isolated from S. chinensis. The differential regulation of S. chinensis B-class MADSbox transcription factors likely plays a role during the development of subtending bracts and perianthless flowers. This study contributes to our understanding of inflorescence development in Saururus, and represents an initial step toward understanding the formation of petaloid bracts in this species.
关键词Floral Organ Identity Mads-box Genes Basal Angiosperms Homeotic Genes Core Eudicots Arabidopsis Expression Evolution Apetala3 Pistillata
资助信息Ministry of Science and Technology of China [2004DKA30430]; National Natural Science Foundation of China [NSFC30860130, NSFC31060166]; Yunnan Natural Science Foundation [2007C209M]; Large-scale Scientific Facilities of the Chinese Academy of Sciences program [2009-LSFGBOWS-01]
学科领域Multidisciplinary Sciences
DOI10.1371/journal.pone.0053019
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000315563800017
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16625
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China
2.Yunnan Agr Univ, Coll Agron, Kunming, Peoples R China
3.Yunnan Agr Univ, Key Lab Plant Pathol Yunnan Prov, Kunming, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China
5.Royal Bot Garden Edinburgh, Edinburgh, Midlothian, Scotland
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Zhao, Yin-He,Larson-Rabin, Zachary,Wang, Guo-Ying,et al. Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis[J]. PLOS ONE,2013,8(1):e53019.
APA Zhao, Yin-He.,Larson-Rabin, Zachary.,Wang, Guo-Ying.,Moeller, Michael.,Li, Cheng-Yun.,...&Li, De-Zhu.(2013).Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis.PLOS ONE,8(1),e53019.
MLA Zhao, Yin-He,et al."Developmental Genetics of the Perianthless Flowers and Bracts of a Paleoherb Species, Saururus chinensis".PLOS ONE 8.1(2013):e53019.
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