High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales

	High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales
	Healy, R. A.1; Smith, M. E.2; Bonito, G. M.3; Pfister, D. H.4; Ge, Z. -W.2,5; Guevara, G. G.; Williams, G.3; Stafford, K.3; Kumar, L.1; Lee, T.1; Hobart, C.6; Trappe, J.7; Vilgalys, R.3; Mclaughlin, D. J.1
通讯作者	Healy, RA (reprint author), Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. ; healy089@umn.edu
	2013-03-01
发表期刊	MOLECULAR ECOLOGY
ISSN	0962-1083
卷号	22 期号:6 页码:1717-1732
摘要	Fungal mitospores may function as dispersal units and/ or spermatia and thus play a role in distribution and/or mating of species that produce them. Mitospore production in ectomycorrhizal (EcM) Pezizales is rarely reported, but here we document mitospore production by a high diversity of EcM Pezizales on three continents, in both hemispheres. We sequenced the internal transcribed spacer (ITS) and partial large subunit (LSU) nuclear rDNA from 292 spore mats (visible mitospore clumps) collected in Argentina, Chile, China, Mexico and the USA between 2009 and 2012. We collated spore mat ITS sequences with 105 fruit body and 47 EcM root sequences to generate operational taxonomic units (OTUs). Phylogenetic inferences were made through analyses of both molecular data sets. A total of 48 OTUs from spore mats represented six independent EcM Pezizales lineages and included truffles and cup fungi. Three clades of seven OTUs have no known meiospore stage. Mitospores failed to germinate on sterile media, or form ectomycorrhizas on Quercus, Pinus and Populus seedlings, consistent with a hypothesized role of spermatia. The broad geographic range, high frequency and phylogenetic diversity of spore mats produced by EcM Pezizales suggests that a mitospore stage is important for many species in this group in terms of mating, reproduction and/or dispersal.
关键词	Cryptic Diversity Ectomycorrhizal Pezizales Environmental Sequencing Mitospore Truffle
学科领域	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000316209500018
引用统计	被引频次：54[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16501
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA 2.Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA 3.Duke Univ, Dept Biol, Durham, NC 27708 USA 4.Harvard Univ, Farlow Herbarium Cryptogam Bot, Cambridge, MA 02143 USA 5.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 6.Univ Sheffield, Sheffield, S Yorkshire, England 7.Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA
推荐引用方式 GB/T 7714	Healy, R. A.,Smith, M. E.,Bonito, G. M.,et al. High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales[J]. MOLECULAR ECOLOGY,2013,22(6):1717-1732.
APA	Healy, R. A..,Smith, M. E..,Bonito, G. M..,Pfister, D. H..,Ge, Z. -W..,...&Mclaughlin, D. J..(2013).High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales.MOLECULAR ECOLOGY,22(6),1717-1732.
MLA	Healy, R. A.,et al."High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales".MOLECULAR ECOLOGY 22.6(2013):1717-1732.

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