Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms

	Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms
	Shi, Chao 1,2,3; Liu, Yuan2,3 ; Huang, Hui 2; Xia, En-Hua2,3 ; Zhang, Hai-Bin 2,3; Gao, Li-Zhi2
通讯作者	Gao, LZ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming, Peoples R China. ; Lgao@mail.kib.ac.cn
	2013-03-18
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:3 页码:e59620
摘要	Plant chloroplast genes are usually co-transcribed while its posttranscriptional splicing is fairly complex and remains largely unsolved. On basis of sequencing the three complete Camellia (Theaceae) chloroplast genomes for the first time, we comprehensively analyzed the evolutionary patterns of ycf15, a plastid gene quite paradoxical in terms of its function and evolution, along the inferred angiosperm phylogeny. Although many species in separate lineages including the three species reported here contained an intact ycf15 gene in their chloroplast genomes, the phylogenetic mixture of both intact and obviously disabled ycf15 genes imply that they are all non-functional. Both intracellular gene transfer (IGT) and horizontal gene transfer (HGT) failed to explain such distributional anomalies. While, transcriptome analyses revealed that ycf15 was transcribed as precursor polycistronic transcript which contained ycf2, ycf15 and antisense trnL-CAA. The transcriptome assembly was surprisingly found to cover near the complete Camellia chloroplast genome. Many non-coding regions including pseudogenes were mapped by multiple transcripts, indicating the generality of pseudogene transcriptions. Our results suggest that plastid DNA posttranscriptional splicing may involve complex cleavage of nonfunctional genes.
关键词	Chloroplast Genome Sequence Complete Nucleotide-sequence Independent Transfers Horizontal Transfer Maximum-likelihood Flowering Plants Rna-metabolism Nucleus Dna Organization
学科领域	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000316411600068
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16493
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Shi, Chao,Liu, Yuan,Huang, Hui,et al. Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms[J]. PLOS ONE,2013,8(3):e59620.
APA	Shi, Chao,Liu, Yuan,Huang, Hui,Xia, En-Hua,Zhang, Hai-Bin,&Gao, Li-Zhi.(2013).Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms.PLOS ONE,8(3),e59620.
MLA	Shi, Chao,et al."Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms".PLOS ONE 8.3(2013):e59620.

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