| Plant cyclopeptide RA-V kills human breast cancer cells by inducing mitochondria-mediated apoptosis through blocking PDK1-AKT interaction | |
| Fang, Xian-Ying1; Chen, Wei1; Fan, Jun-Ting2; Song, Ran1; Wang, Lu1; Gu, Yan-Hong3; Zeng, Guang-Zhi2; Shen, Yan1; Wu, Xue-Feng1; Tan, Ning-Hua2; Xu, Qiang1; Sun, Yang1 | |
| 通讯作者 | Tan, NH (reprint author), Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Peoples R China.，nhtan@mail.kib.ac.cn ; molpharm@163.com ; yangsun@nju.edu.cn |
| 2013-02-15 | |
| 发表期刊 | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| ISSN | 0041-008X |
| 卷号 | 267期号:1页码:95-103 |
| 摘要 | In the present paper, we examined the effects of a natural cyclopeptide RA-V on human breast cancer cells and the underlying mechanisms. RA-V significantly inhibited the growth of human breast cancer MCF-7, MDA-MB-231 cells and murine breast cancer 4T1 cells. In addition, RA-V triggered mitochondria] apoptotic pathway which was indicated by the loss of mitochondrial membrane potential, the release of cytochrome c, and the activation of caspase cascade. Further study showed that RA-V dramatically inhibited phosphorylation of AKT and 3-phosphoinositide dependent protein kinase 1 (PDK1) in MCF-7 cells. Moreover, RA-V disrupted the interaction between PDK1 and AKT in MCF-7 cells. Furthermore, RA-V-induced apoptosis could be enhanced by phosphatidylinositol 3-kinase inhibitor or attenuated by over-expression of AKT in all the three kinds of breast cancer cells. Taken together, this study shows that RA-V, which can induce mitochondria-mediated apoptosis, exerts strong anti-tumor activity against human breast cancer. The underlying anti-cancer mechanism of RA-V is related to the blockage of the interaction between PDK1 and AKT. (C) 2012 Elsevier Inc. All rights reserved. |
| 关键词 | Ra-v Cyclopeptide Mitochondrial Apoptosis Pathway Pi3k/akt Breast Cancer |
| 学科领域 | Pharmacology & Pharmacy ; Toxicology |
| DOI | 10.1016/j.taap.2012.12.010 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314626300010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/16224 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Sch Life Sci, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Peoples R China 3.Nanjing Med Univ, Dept Clin Oncol, Affiliated Hosp 1, Nanjing, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Xian-Ying,Chen, Wei,Fan, Jun-Ting,et al. Plant cyclopeptide RA-V kills human breast cancer cells by inducing mitochondria-mediated apoptosis through blocking PDK1-AKT interaction[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,267(1):95-103. |
| APA | Fang, Xian-Ying.,Chen, Wei.,Fan, Jun-Ting.,Song, Ran.,Wang, Lu.,...&Sun, Yang.(2013).Plant cyclopeptide RA-V kills human breast cancer cells by inducing mitochondria-mediated apoptosis through blocking PDK1-AKT interaction.TOXICOLOGY AND APPLIED PHARMACOLOGY,267(1),95-103. |
| MLA | Fang, Xian-Ying,et al."Plant cyclopeptide RA-V kills human breast cancer cells by inducing mitochondria-mediated apoptosis through blocking PDK1-AKT interaction".TOXICOLOGY AND APPLIED PHARMACOLOGY 267.1(2013):95-103. |
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