露珠杜鹃自然杂交形成机制研究
王年
学位类型硕士
导师张长芹
2010-05
学位授予单位中国科学院研究生院
学位专业植物学
摘要本文通过对露珠杜鹃自然分布范围的调查,研究了其花形态性状在居群内及居群间的变异。通过核糖体和叶绿体片段测序,推测:(1)露珠杜鹃可能是自然杂交起源,其母本为马缨花杜鹃或大白花杜鹃;(2)露珠杜鹃同大白花杜鹃具有相同的叶绿体单倍型,通过和马缨花杜鹃不断的自然杂交,马缨花杜鹃的一些叶绿体基因渗入到了露珠杜鹃之内。主要结果如下:1. 地理分布的调查, 对贵州省大方县百里杜鹃林、沾益县马雄山、师宗县菌子山、漾濞县苍山西坡大坪地、金盏脉地、大理苍山花甸坝以及鹤庆7个地点的杜鹃花资源分布情况进行了调查。结果表明,露珠杜鹃、迷人杜鹃、大白花及马缨花均分布于上述几个地点,且属于同域分布。就分布范围而言,马缨花广泛分布于上述地点。迷人杜鹃在大坪地、花甸坝及鹤庆分布相对较少;露珠杜鹃和大白花杜鹃?从垂直分布看,大白花杜鹃基本分布在海拔较低的区域,马缨花在不同的海拔均广泛分布,而露珠杜鹃和迷人杜鹃海拔总体偏高。2. 不同居群露珠杜鹃的花形态特征数值分析, 通过对露珠杜鹃四个居群的13个花部性状分析表明,除雄蕊数及花瓣裂片数在居群内及居群间无差异外,其余性状均存在不同程度的变异。分布在不同地区4个居群的7个花部性状存在不同程度的相关性。排出地区因素外,28对花部性状组合后,有26对具有显著的相关关系,其中23对花部性状具有及其显著的相关关系。主成分分析表明,雄蕊长和花冠直径两个主成分贡献了52.18%的变异。对四个居群22个个体的花部性状聚类分析表明22个植株主要聚为四个主要的分支,大体代表四个不同的居群。3. 假设亲本马缨花SSR引物开发及通用性检验, 在设计的引物中,有15对具有较好的多态性,每个位点等位基因个数有3-6个,平均有4个。期望的杂合度和实际观察到的杂合度分别为0.0365-0.7091,0.0263-0.9512。七个位点偏离Hardy-Weinberg平衡 (R-111, R-112, R-147, R-299, R-320, R-335, and R-544) (P﹤0.01)。三个位点R-299和R-544, R-166 和R-320, R-111和R-320连锁不平衡。对大白花杜鹃、迷人杜鹃及露珠杜鹃引物种间扩增表明,部分引物适用于亚属内其它种的研究。4. ITS、matK、trnH-psbA及rbcL序列测定, 通过对ITS测序表明,在大白花杜鹃和马缨花杜鹃变异的6个位点,迷人杜鹃为杂合位点,而露珠杜鹃为纯和的,其ITS序列同大白花杜鹃的一致。通过对几个叶绿体片段测序表明,部分露珠杜鹃的叶绿体单倍型同马缨花的一致,另外一些个体的同大白花一致。
资助项目Through investigating sympatric distribution of Rhododendron irroratum, examing the variation of floral characters and sequencing the ITS and other chloroplast segements, we find that (1) R. irroratum might be of hybrid origin, with its maternal parent R. delavayi or R. decorum. (2) The ancestral haplotype of R. irroratum might be identical to that of R. decorum, and it is under ongoing introgression from R. delavayi. 1. The natural distribution, Seven distribution sites of R. irroratum in Guizhou and Yunnan province were investigated. The result shows that R. irroratum is sympatric with R. delavayi, R. decorum and R. agastum. R. delavayi is widespread across the above-mentioned seven sites, whereas R. agastum is scarce in DaPingDi, HuaDianBa and HeQing sites. R. irroratum and R. agastum distribute at the higher elevation compared to that of R. decorum, while R. delavayi is of widespread distribution across both regions of R. decorum and R. irroratum.2. Floral variation of R. irroratum among populations, The floral characters remain vary within and among populations except for the stamen number and the petal number. Seven floral characters correlates with each other among populations, of 28 different combinations, 26 reveal significant correlation, and 23 extremely significant correlation. The PCA analysis shows that the first two components account for 52.18% of the total variation. The dendrogram tree is divided into four main parts, roughly representing the respective populations, which is constructed using 22 R. irroratum individuals. 3. Putative Development and the transferability test of SSR makers, Fifteen microsatellite loci were developed and characterized in R. delavayi. The average allele number of these microsatellites was 4 per locus, ranging from 3 to 6. The ranges of expected (HE) and observed (HO) heterozygosities were 0.0365-0.7091 and 0.0263-0.9512, respectively. Seven loci (R-111, R-112, R-147, R-299, R-320, R-335, and R-544) deviated significantly from the HWE (P﹤0.01). No significant linkage disequilibrium was detected between locus pairs except for three locus pairs: R-299 and R-544, R-166 and R-320, R-111 and R-320. Cross-species amplification in R. agastum, R. decorum, and R. irroratum showed that a subset of these markers holds promise for congeneric species study.4. ITS, matK, trnH-psbA and rbcL sequences. R. delavayi has six sites different from that of R. decorum in its ITS region, whereas R. agastum reveals double peaks at the corresponding sites and R. irroratum is identical to that of R. delavayi. The chloroplast segements show that some R. irroratum individuals share the same haplotype with R. delavayi and others share them with R. decorum.
语种中文
文献类型学位论文
条目标识符http://ir.kib.ac.cn/handle/151853/16140
专题昆明植物所硕博研究生毕业学位论文
推荐引用方式
GB/T 7714
王年. 露珠杜鹃自然杂交形成机制研究[D]. 中国科学院研究生院,2010.
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