Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae)
Cai, Zhao-Ming1,2,3; Zhang, Yu-Xiao1,2; Zhang, Li-Na1,2; Gao, Lian-Ming1; Li, De-Zhu1,2
2012-11-01
发表期刊JOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN1674-4918
卷号50期号:6页码:527-539
摘要Bambusoideae is an important subfamily of the grass family Poaceae that has considerable economic, ecologic and cultural value. In addition, Bambusoideae species are important constituents of the forest vegetation in China. Because of the paucity of flower-bearing specimens and homoplasies of morphological characters, it is difficult to identify species of Bambusoideae using morphology alone, especially in the case of temperate woody bamboos (i.e. Arundinarieae). To this end, DNA barcoding has shown great potential in identifying species. The present study is the first attempt to test the feasibility of four proposed DNA barcoding markers (matK, rbcL, trnH-psbA, and internal transcribed spacer [ITS]) in identifying 27 species of the temperate woody bamboos. Three plastid markers showed high levels of universality, whereas the universality of ITS was comparatively low. A single plastid marker provided low levels of discrimination success at both the genus and species levels (<12%). Among the combinations of plastid markers, the highest discriminatory power was obtained using the combination of rbcL + matK (14.8%). Using a combination of three markers did not increase species discrimination. The nuclear region ITS alone could identify 66.7% of species, although fewer taxa were included in the ITS analyses than in the plastid analyses. When ITS was integrated with a single or combination of plastid markers, the species discriminatory power was significantly improved. We suggest that a combination of rbcL + ITS, which exhibited the highest species identification power of all combinations in the present study, could be used as a potential DNA barcode for temperate woody bamboos.
关键词Dna Barcoding Internal Transcribed Spacer (Its) Matk Rbcl Temperate Woody Bamboos
资助信息Research Fund for the Large-Scale Scientific Facilities of the Chinese Academy of Sciences [2009-LSF-GBOWS-01]; National Natural Science Foundation of China [U1136603, 30990244]; talent project of the Yunnan Province of China [2008YP064]; program of innovation teams of Yunnan Province of China [392009CI011]
学科领域Plant Sciences
DOI10.1111/j.1759-6831.2012.00216.x
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000310888700007
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15966
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Gemplasm & Genom Ctr, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Cai, Zhao-Ming,Zhang, Yu-Xiao,Zhang, Li-Na,et al. Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2012,50(6):527-539.
APA Cai, Zhao-Ming,Zhang, Yu-Xiao,Zhang, Li-Na,Gao, Lian-Ming,&Li, De-Zhu.(2012).Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae).JOURNAL OF SYSTEMATICS AND EVOLUTION,50(6),527-539.
MLA Cai, Zhao-Ming,et al."Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 50.6(2012):527-539.
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