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题名: A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS
作者: Yang, Han-Qi1; Dong, Yu-Ran1; Gu, Zhi-Jia1; Liang, Ning1; Yang, Jun-Bo2
刊名: ANNALES BOTANICI FENNICI
关键词: MOLECULAR PHYLOGENETICS ; FLOWERING PLANTS ; SEQUENCE DATA ; LAND PLANTS ; PLASTID DNA ; IDENTIFICATION ; PALMAE ; SUBSTITUTION ; CALAMOIDEAE ; TAXONOMY
英文摘要: Calamus is the largest genus in the palm family (Arecaceae) and contains many species of high ecological and economical value. In this study, we assessed the nuclear ribosomal internal transcribed spacer region (ITS), matK, rbcL, trnH-psbA, as well as two combinations, matK + rbcL and matK + rbcL + trnH-psbA, as DNA barcodes for Calamus using 15 species or varieties distributed in China. ITS may exist as multiple copies in the examined Calamus species, and was eliminated from consideration as a possible barcode. The trnH-psbA spacer had the most variation, followed by matK and rbcL. No separations between intraspecific variation and interspecific divergence (barcoding gaps) were found in the remaining candidate barcodes. At the species level, the discrimination rates of the candidate barcodes based on neighbor-joining (NJ) trees were significantly different: matK (37.5%), rbcL (6.3%), trnH-psbA (56.3%), matK + rbcL (43.8%) and matK + rbcL + trnH-psbA (62.5%). Furthermore, the discrimination rates of trnH-psbA would improve to 91.7%, if the varieties of C. nambariensis and C. yunnanensis were treated as one species in the NJ tree. Thus, trnH-psbA may be an appropriate single DNA barcode for Calamus useful in the species identification.
出版日期: 2012-11-30
卷号: 49, 期号:5-6, 页码:319-330
语种: 英语
收录类别: SCI
学科分类: Plant Sciences
ISSN号: 0003-3847
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/15962
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China

Recommended Citation:
Yang, Han-Qi; Dong, Yu-Ran; Gu, Zhi-Jia; Liang, Ning; Yang, Jun-Bo.A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS,ANNALES BOTANICI FENNICI,2012,49(5-6):319-330
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