How well do ITS rDNA sequences differentiate species of true morels (Morchella)?

doi:10.3852/12-056

	How well do ITS rDNA sequences differentiate species of true morels (Morchella)?
	Du, Xi-Hui 10,11; Zhao, Qi10,11 ; Yang, Zhu L.10,11 ; Hansen, Karen 9; Taskin, Hatira 8; Buyukalaca, Saadet 8; Dewsbury, Damon 6,7; Moncalvo, Jean-Marc 6,7; Douhan, Greg W.5; Robert, Vincent A. R. G.4; Crous, Pedro W.4; Rehner, Stephen A.3; Rooney, Alejandro P.2; Sink, Stacy 1; O'Donnell, Kerry 1
	2012-11-01
发表期刊	MYCOLOGIA
卷号	104 期号:6 页码:1351-1368
摘要	Arguably more mycophiles hunt true morels (Morchella) during their brief fruiting season each spring in the northern hemisphere than any other wild edible fungus. Concerns about overharvesting by individual collectors and commercial enterprises make it essential that science-based management practices and conservation policies are developed to ensure the sustainability of commercial harvests and to protect and preserve morel species diversity. Therefore, the primary objectives of the present study were to: (i) investigate the utility of the ITS rDNA locus for identifying Morchella species, using phylogenetic species previously inferred from multilocus DNA sequence data as a reference; and (ii) clarify insufficiently identified sequences and determine whether the named sequences in GenBank were identified correctly. To this end, we generated 553 Morchella ITS rDNA sequences and downloaded 312 additional ones generated by other researchers from GenBank using emerencia and analyzed them phylogenetically. Three major findings emerged: (i) ITS rDNA sequences were useful in identifying 48/62 (77.4%) of the known phylospecies; however, they failed to identify 12 of the 22 species within the species-rich Elata Subclade and two closely related species in the Esculenta Clacle; (ii) at least 66% of the named Morchella sequences in GenBank are misidentified; and (iii) ITS rDNA sequences of up to six putatively novel Morchella species were represented in GenBank. Recognizing the need for a dedicated Web-accessible reference database to facilitate the rapid identification of known and novel species, we constructed Morchella MLST (http://www.cbs.knaw.nl/morchella/), which can be queried with ITS rDNA sequences and those of the four other genes used in our prior multilocus molecular systematic studies of this charismatic genus.
关键词	Ascomycota Biodiversity Biogeography Emerencia Gcpsr Genbank Phylogeny Species Limits
DOI	10.3852/12-056
收录类别	SCI
语种	英语
WOS记录号	WOS:000312361100009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/15798
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA 2.USDA ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA 3.USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA 4.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands 5.Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA 6.Royal Ontario Museum, Dept Nat Hist, Toronto, ON M5S 2C6, Canada 7.Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 2C6, Canada 8.Cukurova Univ, Fac Agr, Dept Hort, TR-01330 Adana, Turkey 9.Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden 10.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 11.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan Province, Peoples R China
推荐引用方式 GB/T 7714	Du, Xi-Hui,Zhao, Qi,Yang, Zhu L.,et al. How well do ITS rDNA sequences differentiate species of true morels (Morchella)?[J]. MYCOLOGIA,2012,104(6):1351-1368.
APA	Du, Xi-Hui.,Zhao, Qi.,Yang, Zhu L..,Hansen, Karen.,Taskin, Hatira.,...&O'Donnell, Kerry.(2012).How well do ITS rDNA sequences differentiate species of true morels (Morchella)?.MYCOLOGIA,104(6),1351-1368.
MLA	Du, Xi-Hui,et al."How well do ITS rDNA sequences differentiate species of true morels (Morchella)?".MYCOLOGIA 104.6(2012):1351-1368.

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