The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.

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	The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.
	Zhou, X ; Tan, DY ; Lai, JH ; Zan, RG
	1998
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	25 期号:5 页码:472-475
摘要	A method for RT-PCR amplifying long fragments cDNA was established and the 7.4 kb full-length cDNA of Hepatitis A virus (HAV) H-2 strain was amplifyed by reverse transcription and polymerase chain reaction (RT-PCR). HAV H-2 was precipitated by anti-H-2 serum specifically, then the RNA of HAV H-2 was isolated from this precipitation by acid guanidinium hydrochloride-phenal-chloroform extraction. The first-strand of HAV H-2 cDNA was synthesised by reverse transcriptase without RNase H activity, then was amplified by PCR using the 32mer primer and the Tag DNA polymerase with Deep Vent DNA polymerase. For obtaining longer PCR products, it is necessary to prepare high quality RNA and employ the longer primers and special Tag DNA polymerase.
关键词	Hepatitis a Virus H-2 Strain Full Length Rna Rt-pcr Amplification
学科领域	Biochemistry & Molecular Biology ; Biophysics
收录类别	sci
语种	中文
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/15576
专题	离退休
推荐引用方式 GB/T 7714	Zhou, X,Tan, DY,Lai, JH,et al. The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,1998,25(5):472-475.
APA	Zhou, X,Tan, DY,Lai, JH,&Zan, RG.(1998).The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,25(5),472-475.
MLA	Zhou, X,et al."The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 25.5(1998):472-475.

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