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The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.
Zhou, X ; Tan, DY ; Lai, JH ; Zan, RG
1998
发表期刊PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
卷号25期号:5页码:472-475
摘要A method for RT-PCR amplifying long fragments cDNA was established and the 7.4 kb full-length cDNA of Hepatitis A virus (HAV) H-2 strain was amplifyed by reverse transcription and polymerase chain reaction (RT-PCR). HAV H-2 was precipitated by anti-H-2 serum specifically, then the RNA of HAV H-2 was isolated from this precipitation by acid guanidinium hydrochloride-phenal-chloroform extraction. The first-strand of HAV H-2 cDNA was synthesised by reverse transcriptase without RNase H activity, then was amplified by PCR using the 32mer primer and the Tag DNA polymerase with Deep Vent DNA polymerase. For obtaining longer PCR products, it is necessary to prepare high quality RNA and employ the longer primers and special Tag DNA polymerase.
关键词Hepatitis a Virus H-2 Strain Full Length Rna Rt-pcr Amplification
学科领域Biochemistry & Molecular Biology ; Biophysics
收录类别sci
语种中文
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15576
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GB/T 7714
Zhou, X,Tan, DY,Lai, JH,et al. The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,1998,25(5):472-475.
APA Zhou, X,Tan, DY,Lai, JH,&Zan, RG.(1998).The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,25(5),472-475.
MLA Zhou, X,et al."The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 25.5(1998):472-475.
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