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The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.
Zhou, X ; Tan, DY ; Lai, JH ; Zan, RG
1998
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume25Issue:5Pages:472-475
AbstractA method for RT-PCR amplifying long fragments cDNA was established and the 7.4 kb full-length cDNA of Hepatitis A virus (HAV) H-2 strain was amplifyed by reverse transcription and polymerase chain reaction (RT-PCR). HAV H-2 was precipitated by anti-H-2 serum specifically, then the RNA of HAV H-2 was isolated from this precipitation by acid guanidinium hydrochloride-phenal-chloroform extraction. The first-strand of HAV H-2 cDNA was synthesised by reverse transcriptase without RNase H activity, then was amplified by PCR using the 32mer primer and the Tag DNA polymerase with Deep Vent DNA polymerase. For obtaining longer PCR products, it is necessary to prepare high quality RNA and employ the longer primers and special Tag DNA polymerase.
KeywordHepatitis a Virus H-2 Strain Full Length Rna Rt-pcr Amplification
Subject AreaBiochemistry & Molecular Biology ; Biophysics
Indexed Bysci
Language中文
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/15576
Collection离退休
Recommended Citation
GB/T 7714
Zhou, X,Tan, DY,Lai, JH,et al. The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,1998,25(5):472-475.
APA Zhou, X,Tan, DY,Lai, JH,&Zan, RG.(1998).The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,25(5),472-475.
MLA Zhou, X,et al."The RT-PCR amplification of full length RNA of hepatitis a virus H-2 strain.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 25.5(1998):472-475.
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