Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene

doi:10.1080/10425170600699877

	Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene
	Tian, Bo ; Chen, Yongyan ; Li, Dezhu; Yan, Yuanxin
通讯作者	Li, DZ (reprint author), Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China. tianbo@mail.kib.ac.cn ; dlchyy@126.com ; dzl@mail.kib.ac.cn ; Yanyuanxin@mail.kib.ac.cn
	2006-04-01
发表期刊	DNA SEQUENCE
ISSN	1042-5179
卷号	17 期号:2 页码:143-151
摘要	A cDNA named DlMADS8 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by rapid amplification of cDNA end (RACE). DNA sequence analysis showed that DlMADS8 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1059 nucleotides and encoded a putative protein of 244 amino acid residues. The gene displayed the structure of a typical plant MADS-box gene, which consisted of a MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS-box genes based on amino acid sequences revealed that DlMADS8 was grouped into the AGAMOUS-LIKE 2 (AGL2)-like subfamily. It was homologous to the LEAFY HULL STERILE1 (LHS1) genes of grasses. To study the functions of it, DIMADS8 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis thaliana. Transgenic plants of DIMADS8 exhibited the phenotypes of curled leaves and early flowering. After bolting, three novel phenotypes related to inflorescence development were observed in different transgenic plants. No obvious homeotic conversions of floral organs were observed in all of the 35S::DlMADS8 transgenic Arabidopsis plants. These results indicated that DlMADS8 probably plays a role in floral meristem determinacy and is involved in controlling the flowering time of D. latiflorus.
关键词	Dendrocalamus Latiflorus Mads-box Gene Agl2-like Leafy Hull Sterile1
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1080/10425170600699877
收录类别	SCI
语种	英语
WOS记录号	WOS:000240356000009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/15560
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
推荐引用方式 GB/T 7714	Tian, Bo,Chen, Yongyan,Li, Dezhu,et al. Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene[J]. DNA SEQUENCE,2006,17(2):143-151.
APA	Tian, Bo,Chen, Yongyan,Li, Dezhu,&Yan, Yuanxin.(2006).Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene.DNA SEQUENCE,17(2),143-151.
MLA	Tian, Bo,et al."Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene".DNA SEQUENCE 17.2(2006):143-151.

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