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Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene
Tian, Bo; Chen, Yongyan; Li, Dezhu; Yan, Yuanxin
Corresponding AuthorLi, DZ (reprint author), Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China. tianbo@mail.kib.ac.cn ; dlchyy@126.com ; dzl@mail.kib.ac.cn ; Yanyuanxin@mail.kib.ac.cn
2006-04-01
Source PublicationDNA SEQUENCE
ISSN1042-5179
Volume17Issue:2Pages:143-151
AbstractA cDNA named DlMADS8 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by rapid amplification of cDNA end (RACE). DNA sequence analysis showed that DlMADS8 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1059 nucleotides and encoded a putative protein of 244 amino acid residues. The gene displayed the structure of a typical plant MADS-box gene, which consisted of a MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS-box genes based on amino acid sequences revealed that DlMADS8 was grouped into the AGAMOUS-LIKE 2 (AGL2)-like subfamily. It was homologous to the LEAFY HULL STERILE1 (LHS1) genes of grasses. To study the functions of it, DIMADS8 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis thaliana. Transgenic plants of DIMADS8 exhibited the phenotypes of curled leaves and early flowering. After bolting, three novel phenotypes related to inflorescence development were observed in different transgenic plants. No obvious homeotic conversions of floral organs were observed in all of the 35S::DlMADS8 transgenic Arabidopsis plants. These results indicated that DlMADS8 probably plays a role in floral meristem determinacy and is involved in controlling the flowering time of D. latiflorus.
KeywordDendrocalamus Latiflorus Mads-box Gene Agl2-like Leafy Hull Sterile1
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
DOI10.1080/10425170600699877
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000240356000009
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/15560
Collection中国西南野生生物种质资源库
Affiliation1.Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
Recommended Citation
GB/T 7714
Tian, Bo,Chen, Yongyan,Li, Dezhu,et al. Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene[J]. DNA SEQUENCE,2006,17(2):143-151.
APA Tian, Bo,Chen, Yongyan,Li, Dezhu,&Yan, Yuanxin.(2006).Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene.DNA SEQUENCE,17(2),143-151.
MLA Tian, Bo,et al."Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene".DNA SEQUENCE 17.2(2006):143-151.
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