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Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene
Tian, Bo; Chen, Yongyan; Li, Dezhu; Yan, Yuanxin
通讯作者Li, DZ (reprint author), Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China. tianbo@mail.kib.ac.cn ; dlchyy@126.com ; dzl@mail.kib.ac.cn ; Yanyuanxin@mail.kib.ac.cn
2006-04-01
发表期刊DNA SEQUENCE
ISSN1042-5179
卷号17期号:2页码:143-151
摘要A cDNA named DlMADS8 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by rapid amplification of cDNA end (RACE). DNA sequence analysis showed that DlMADS8 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1059 nucleotides and encoded a putative protein of 244 amino acid residues. The gene displayed the structure of a typical plant MADS-box gene, which consisted of a MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS-box genes based on amino acid sequences revealed that DlMADS8 was grouped into the AGAMOUS-LIKE 2 (AGL2)-like subfamily. It was homologous to the LEAFY HULL STERILE1 (LHS1) genes of grasses. To study the functions of it, DIMADS8 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis thaliana. Transgenic plants of DIMADS8 exhibited the phenotypes of curled leaves and early flowering. After bolting, three novel phenotypes related to inflorescence development were observed in different transgenic plants. No obvious homeotic conversions of floral organs were observed in all of the 35S::DlMADS8 transgenic Arabidopsis plants. These results indicated that DlMADS8 probably plays a role in floral meristem determinacy and is involved in controlling the flowering time of D. latiflorus.
关键词Dendrocalamus Latiflorus Mads-box Gene Agl2-like Leafy Hull Sterile1
学科领域Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI10.1080/10425170600699877
收录类别SCI
语种英语
WOS记录号WOS:000240356000009
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15560
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China
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Tian, Bo,Chen, Yongyan,Li, Dezhu,et al. Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene[J]. DNA SEQUENCE,2006,17(2):143-151.
APA Tian, Bo,Chen, Yongyan,Li, Dezhu,&Yan, Yuanxin.(2006).Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene.DNA SEQUENCE,17(2),143-151.
MLA Tian, Bo,et al."Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene".DNA SEQUENCE 17.2(2006):143-151.
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