Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings

doi:10.1016/j.plaphy.2012.04.003

	Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings
	Deng, Wei-Wei 1,2; Wang, Shuo 1,2; Chen, Qi 1,2; Zhang, Zheng-Zhu 1,2; Hu, Xiang-Yang 3
通讯作者	Zhang, ZZ (reprint author), Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, 130 Changjiang W Rd, Hefei 230036, Anhui, Peoples R China. ; zzz@ahau.edu.cn
	2012-07-01
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	56 期号:1 页码:35-40
摘要	Theanine synthetase (TS) is an enzyme involved in theanine biosynthesis in tea plants. Recent studies have revealed that theanine biosynthesis, derived from nitrogen metabolism in tea (Camellia sinensis plants, could be influenced by salt treatment. We have characterized CsTS at the molecular and biochemical level. The expression pattern of CsTS protein was examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of salt treatment on the levels of theanine synthesis was investigated in this study. Levels of theanine and the total free amino acids were gradually increased in shoots, and reached the maximum on the 8th day after treatment (DAT). The immunoblotting analysis suggested the accumulation of CsTS protein had increased gently up to 8 DAT, and subsequently declined, both in roots and shoots, which is one of the main evidences that resulted in the variation of theanine concentration under salt treatment. Together, these data revealed that theanine synthesis takes place both in root and shoot and CsTS accumulation is positively affected by salt treatment. (C) 2012 Elsevier Masson SAS. All rights reserved.
关键词	Camellia Sinensis Theanine Synthetase Salt Theanine Amino Acid
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2012.04.003
收录类别	SCI
语种	英语
WOS记录号	WOS:000305779600004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/15117
专题	中国科学院青藏高原研究所昆明部
作者单位	1.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Anhui, Peoples R China 2.Anhui Agr Univ, Minist Agr, Hefei 230036, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Deng, Wei-Wei,Wang, Shuo,Chen, Qi,et al. Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2012,56(1):35-40.
APA	Deng, Wei-Wei,Wang, Shuo,Chen, Qi,Zhang, Zheng-Zhu,&Hu, Xiang-Yang.(2012).Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings.PLANT PHYSIOLOGY AND BIOCHEMISTRY,56(1),35-40.
MLA	Deng, Wei-Wei,et al."Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings".PLANT PHYSIOLOGY AND BIOCHEMISTRY 56.1(2012):35-40.

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