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题名: Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings
作者: Deng, Wei-Wei1, 2; Wang, Shuo1, 2; Chen, Qi1, 2; Zhang, Zheng-Zhu1, 2; Hu, Xiang-Yang3
刊名: PLANT PHYSIOLOGY AND BIOCHEMISTRY
关键词: Camellia sinensis ; Theanine synthetase ; Salt ; Theanine ; Amino acid
英文摘要: Theanine synthetase (TS) is an enzyme involved in theanine biosynthesis in tea plants. Recent studies have revealed that theanine biosynthesis, derived from nitrogen metabolism in tea (Camellia sinensis plants, could be influenced by salt treatment. We have characterized CsTS at the molecular and biochemical level. The expression pattern of CsTS protein was examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of salt treatment on the levels of theanine synthesis was investigated in this study. Levels of theanine and the total free amino acids were gradually increased in shoots, and reached the maximum on the 8th day after treatment (DAT). The immunoblotting analysis suggested the accumulation of CsTS protein had increased gently up to 8 DAT, and subsequently declined, both in roots and shoots, which is one of the main evidences that resulted in the variation of theanine concentration under salt treatment. Together, these data revealed that theanine synthesis takes place both in root and shoot and CsTS accumulation is positively affected by salt treatment. (C) 2012 Elsevier Masson SAS. All rights reserved.
出版日期: 2012-07-01
卷号: 56, 期号:1, 页码:35-40
DOI标识: 10.1016/j.plaphy.2012.04.003
语种: 英语
ISSN号: 0981-9428
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/15117
Appears in Collections:中国科学院青藏高原研究所昆明部_期刊论文

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作者单位: 1.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Anhui, Peoples R China
2.Anhui Agr Univ, Minist Agr, Hefei 230036, Anhui, Peoples R China
3.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China

Recommended Citation:
Deng, Wei-Wei; Wang, Shuo; Chen, Qi; Zhang, Zheng-Zhu; Hu, Xiang-Yang.Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings,PLANT PHYSIOLOGY AND BIOCHEMISTRY,2012,56():35-40
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