Purification, crystallization and preliminary X-ray diffraction analysis of a novel mannose-binding lectin from Gastrodia elata with antifungal properties

doi:10.1107/S0907444902014701

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	Purification, crystallization and preliminary X-ray diffraction analysis of a novel mannose-binding lectin from Gastrodia elata with antifungal properties
	Liu, W ; Hu, YL ; Wang, M ; Xiang, Y ; Hu, Z ; Wang, DC
	2002-10-01
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN	0907-4449
卷号	58 期号:Part 10 Sp. Iss. 2 页码:1833-1835
摘要	A plant antifungal protein, Gastrodia antifungal protein (GAFP-1) has been isolated from terminal corms of the orchid Gastrodia elata B1 f. elata, purified to homogeneity and crystallized by means of the hanging-drop vapour-diffusion method. The best quality crystals grew over several months at 277 K. The crystal used for data collection belongs to the space group P2(1)2(1)2, with unit-cell parameters a = 61.087, b = 91.488, c = 81.132 Angstrom. Using a synchrotron-radiation source, the resolution limit of the data reached 2.0 Angstrom, with an overall R-merge of 0.097 and a completeness of 99.8%. Four independent molecules were estimated to be present in the asymmetric unit, with a solvent content of 46.3%. This data will help to solve the first structure of a monomeric monocot mannose-binding lectin.
学科领域	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
DOI	10.1107/S0907444902014701
收录类别	SCI
语种	英语
WOS记录号	WOS:000178248100011
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/14381
专题	离退休
作者单位	1.Chinese Acad Sci, Inst Biophys, Ctr Mol Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Liu, W,Hu, YL,Wang, M,et al. Purification, crystallization and preliminary X-ray diffraction analysis of a novel mannose-binding lectin from Gastrodia elata with antifungal properties[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2002,58(Part 10 Sp. Iss. 2):1833-1835.
APA	Liu, W,Hu, YL,Wang, M,Xiang, Y,Hu, Z,&Wang, DC.(2002).Purification, crystallization and preliminary X-ray diffraction analysis of a novel mannose-binding lectin from Gastrodia elata with antifungal properties.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,58(Part 10 Sp. Iss. 2),1833-1835.
MLA	Liu, W,et al."Purification, crystallization and preliminary X-ray diffraction analysis of a novel mannose-binding lectin from Gastrodia elata with antifungal properties".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 58.Part 10 Sp. Iss. 2(2002):1833-1835.

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