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A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning
Shao, F; Hu, Z; Xiong, YM; Huang, QZ; Wang, CG; Zhu, RH; Wang, DC
Corresponding Authorwdc@pewdc.ibp.ac.cn
1999-03-19
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
ISSN0167-4838
Volume1430Issue:2Pages:262-268
AbstractAn antifungal peptide from seeds of Phytolacca americana, designated PAFP-s, has been isolated. The peptide is highly basic and consists of 38 residues with three disulfide bridges. Its molecular mass of 3929.0 was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation, and cDNA cloning was successfully performed by 3'-RACE. The deduced amino acid sequence of a partial cDNA corresponded to the amino acid sequence from chemical sequencing. PAFP-s exhibited a broad spectrum of antifungal activity, and its activities differed among various fungi. PAFP-s displayed no inhibitory activity towards Escherichia coli. PAFP-s shows significant sequence similarities and the same cysteine motif with Mj-AMPs, antimicrobial peptides from seeds of Mirabilis jalapa belonging to the knottin-type antimicrobial peptide. (C) 1999 Elsevier Science B.V. All rights reserved.
KeywordAntifungal Peptide Knottin-like Peptide Amino Acid Sequence Cdna Cloning
Subject AreaBiochemistry & Molecular Biology ; Biophysics
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000079411700010
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/14367
Collection离退休
Affiliation1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
Recommended Citation
GB/T 7714
Shao, F,Hu, Z,Xiong, YM,et al. A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1999,1430(2):262-268.
APA Shao, F.,Hu, Z.,Xiong, YM.,Huang, QZ.,Wang, CG.,...&Wang, DC.(1999).A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1430(2),262-268.
MLA Shao, F,et al."A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1430.2(1999):262-268.
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