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A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning
Shao, F; Hu, Z; Xiong, YM; Huang, QZ; Wang, CG; Zhu, RH; Wang, DC
1999-03-19
发表期刊BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
ISSN0167-4838
卷号1430期号:2页码:262-268
摘要An antifungal peptide from seeds of Phytolacca americana, designated PAFP-s, has been isolated. The peptide is highly basic and consists of 38 residues with three disulfide bridges. Its molecular mass of 3929.0 was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation, and cDNA cloning was successfully performed by 3'-RACE. The deduced amino acid sequence of a partial cDNA corresponded to the amino acid sequence from chemical sequencing. PAFP-s exhibited a broad spectrum of antifungal activity, and its activities differed among various fungi. PAFP-s displayed no inhibitory activity towards Escherichia coli. PAFP-s shows significant sequence similarities and the same cysteine motif with Mj-AMPs, antimicrobial peptides from seeds of Mirabilis jalapa belonging to the knottin-type antimicrobial peptide. (C) 1999 Elsevier Science B.V. All rights reserved.
关键词Antifungal Peptide Knottin-like Peptide Amino Acid Sequence Cdna Cloning
学科领域Biochemistry & Molecular Biology ; Biophysics
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000079411700010
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14367
专题离退休
作者单位1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式
GB/T 7714
Shao, F,Hu, Z,Xiong, YM,et al. A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1999,1430(2):262-268.
APA Shao, F.,Hu, Z.,Xiong, YM.,Huang, QZ.,Wang, CG.,...&Wang, DC.(1999).A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1430(2),262-268.
MLA Shao, F,et al."A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1430.2(1999):262-268.
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