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题名: A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning
作者: Shao, F; Hu, Z; Xiong, YM; Huang, QZ; Wang, CG; Zhu, RH; Wang, DC
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
关键词: antifungal peptide ; knottin-like peptide ; amino acid sequence ; cDNA cloning
英文摘要: An antifungal peptide from seeds of Phytolacca americana, designated PAFP-s, has been isolated. The peptide is highly basic and consists of 38 residues with three disulfide bridges. Its molecular mass of 3929.0 was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation, and cDNA cloning was successfully performed by 3'-RACE. The deduced amino acid sequence of a partial cDNA corresponded to the amino acid sequence from chemical sequencing. PAFP-s exhibited a broad spectrum of antifungal activity, and its activities differed among various fungi. PAFP-s displayed no inhibitory activity towards Escherichia coli. PAFP-s shows significant sequence similarities and the same cysteine motif with Mj-AMPs, antimicrobial peptides from seeds of Mirabilis jalapa belonging to the knottin-type antimicrobial peptide. (C) 1999 Elsevier Science B.V. All rights reserved.
出版日期: 1999-03-19
卷号: 1430, 期号:2, 页码:262-268
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Biophysics
ISSN号: 0167-4838
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14367
Appears in Collections:离退休_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China

Recommended Citation:
Shao, F; Hu, Z; Xiong, YM; Huang, QZ; Wang, CG; Zhu, RH; Wang, DC.A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning,BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1999,1430(2):262-268
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