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题名: Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv
作者: Huang, RH; Xiang, Y; Liu, XZ; Zhang, Y; Hu, Z; Wang, DC
刊名: FEBS LETTERS
关键词: antifungal peptide ; amino acid sequence ; disulfide motif ; Eucommia ulmoides Oliv
英文摘要: Two antifungal peptides, named EAFP1 and EAFP2, have been purified from the bark of Eucommia ulmoides Oliv. Each of the sequences consists of 41 residues with a N-terminal blockage by pyroglutamic acid determined by automated Edman degradation in combination with the tandem mass spectroscopy and the C-terminal ladder sequencing analysis. The primary structurs all contain 10 cysteines, which are cross-linked to form five disulfide bridges with a pairing pattern (C1-C5, C2-C9, C3-C6, C4-C7, C8-C10). This is the first finding of a plant antifungal peptide with a five-disulfide motif. EAFP1 and EAFP2 show characteristics of hevein domain and exhibit chitin-binding properties similar to the previously identified hevein-like peptides. They exhibit relatively broad spectra of antifungal activities against eight pathogenic fungi from cotton, wheat, potato, tomato and tobacco. The inhibition activity of EAFP1 and EAFP2 can be effective on both chitin-containing and chitin-free fungi. The values of IC50 range from 35 to 155 mug/ml for EAFP1 and 18 to 109 mug/ml for EAFP2. Their antifungal effects are strongly antagonized by calcium ions. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
出版日期: 2002-06-19
卷号: 521, 期号:1-3, 页码:87-90
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
ISSN号: 0014-5793
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14357
Appears in Collections:离退休_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Biophys, Ctr Mol Biol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China

Recommended Citation:
Huang, RH,Xiang, Y,Liu, XZ,et al. Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv[J]. FEBS LETTERS,2002,521(1-3):87-90.
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