Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm

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	Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm
	Zeng, ZH ; Jin, L ; Li, HM ; Hu, Z ; Wang, DC
	1998-08-01
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	41 期号:4 页码:413-418
摘要	Crystals of pokeweed antiviral protein (PAP) from seeds of Phytolacca americana with high diffraction ability were grown from high protein concentration (100 mg/mL) solution at high temperature (33 degrees C). The crystal structure was solved by use of molecular replacement method and refined by use of molecular dynamic method at 0.25 nm to an R factor of 18.15% with standard deviations from standard geometry of 0.001 6 nm and 2.04 degrees for bond lengths and bond angles, respectively. Comparison with two other PAPs revealed, near the active center, a sequence and structure-variable region, consisting of the loop connecting the fifth beta-strand with the second a-helix and including a proposed active residue, suggesting this loop probably to be related to difference in activity.
关键词	Pokeweed Antiviral Protein Ribosome Inactivating Protein Crystal Structure
学科领域	Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000075306700012
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/14269
专题	离退休
作者单位	1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Zeng, ZH,Jin, L,Li, HM,et al. Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1998,41(4):413-418.
APA	Zeng, ZH,Jin, L,Li, HM,Hu, Z,&Wang, DC.(1998).Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,41(4),413-418.
MLA	Zeng, ZH,et al."Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 41.4(1998):413-418.

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