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Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm
Zeng, ZH; Jin, L; Li, HM; Hu, Z; Wang, DC
1998-08-01
发表期刊SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN1006-9305
卷号41期号:4页码:413-418
摘要Crystals of pokeweed antiviral protein (PAP) from seeds of Phytolacca americana with high diffraction ability were grown from high protein concentration (100 mg/mL) solution at high temperature (33 degrees C). The crystal structure was solved by use of molecular replacement method and refined by use of molecular dynamic method at 0.25 nm to an R factor of 18.15% with standard deviations from standard geometry of 0.001 6 nm and 2.04 degrees for bond lengths and bond angles, respectively. Comparison with two other PAPs revealed, near the active center, a sequence and structure-variable region, consisting of the loop connecting the fifth beta-strand with the second a-helix and including a proposed active residue, suggesting this loop probably to be related to difference in activity.
关键词Pokeweed Antiviral Protein Ribosome Inactivating Protein Crystal Structure
学科领域Biology
收录类别SCI
语种英语
WOS研究方向Life Sciences & Biomedicine - Other Topics
WOS类目Biology
WOS记录号WOS:000075306700012
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14269
专题离退休
作者单位1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式
GB/T 7714
Zeng, ZH,Jin, L,Li, HM,et al. Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1998,41(4):413-418.
APA Zeng, ZH,Jin, L,Li, HM,Hu, Z,&Wang, DC.(1998).Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,41(4),413-418.
MLA Zeng, ZH,et al."Crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana at 0.25 nm".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 41.4(1998):413-418.
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