中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 离退休  > 期刊论文
题名: Solution structure of PAFP-S: A new knottin-type antifungal peptide from the seeds of Phytolacca americana
作者: Gao, GH; Liu, W; Dai, JX; Wang, JF; Hu, Z; Zhang, Y; Wang, DC
刊名: BIOCHEMISTRY
英文摘要: The three-dimensional solution structure of PAFP-S, an antifungal peptide extracted from the seeds of Phytolacca americana, was determined using H-1 NMR spectroscopy. This cationic peptide contains 38 amino acid residues. Its structure was determined from 302 distance restraints and 36 dihedral restraints derived from NOEs and coupling constants. The peptide has six cysteines involved in three disulfide bonds. The previously unassigned parings have now been determined from NMR data. The solution structure of PAFP-S is presented as a set of 20 structures using ab initio dynamic simulated annealing, with an average RMS deviation of 1.68 Angstrom for the backbone heavy atoms and 2.19 Angstrom for all heavy atoms, respectively. For the well-defined triple-stranded beta -sheet involving residues 8-10, 23-27, and 32-36, the corresponding values were 0.39 and 1.25 Angstrom. The global fold involves a cystine-knotted three-stranded antiparallel beta -sheet (residues 8-10, 23-27, 32-36), a flexible loop (residues 14-19), and four beta -reverse turns (residues 4-8, 11-14, 19-22, 28-32). This structure features all the characteristics of the knottin fold. It is the first structural model of an antifungal peptide that adopts a knottin-type structure. PAFP-S has an extended hydrophobic surface comprised of residues Tyr23, Phe25, Ile27, Tyr32, and Val34. The side chains of these residues are well-defined in the NMR structure. Several hydrophilic and positively charged residues (Arg9, Arg38, and Lys36) surround the hydrophobic surface, giving PAFP-S an amphiphilic character which would be the main structural basis of its biological function.
出版日期: 2001-09-18
卷号: 40, 期号:37, 页码:10973-10978
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology
ISSN号: 0006-2960
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14189
Appears in Collections:离退休_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
BIOCHEMISTRY40 10973-10978.pdf(11003KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Lab Mol Biophys, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China

Recommended Citation:
Gao, GH; Liu, W; Dai, JX; Wang, JF; Hu, Z; Zhang, Y; Wang, DC.Solution structure of PAFP-S: A new knottin-type antifungal peptide from the seeds of Phytolacca americana,BIOCHEMISTRY,2001,40(37):10973-10978
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, GH]'s Articles
[Liu, W]'s Articles
[Dai, JX]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, GH]‘s Articles
[Liu, W]‘s Articles
[Dai, JX]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: BIOCHEMISTRY40 10973-10978.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace