| Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort) | |
Huang, Pan-Hui1,2; Yu, Wen-Bin1,2; Yang, Jun-Bo3; Wang, Hong1 ; Lu, Lu1
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| 2010-07-01 | |
| 发表期刊 | HORTSCIENCE
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| 卷号 | 45期号:7页码:1129-1131 |
| 摘要 | Pedicularis rex C. B. Clarke ex Maxim., an endemic species with potential horticultural traits from Himalaya, has a unique cup-like petiole structure and highly infraspecific floral variation among members of the lousewort genus (Orobanchaceae). We developed 13 microsatellite markers from three microsatellite-enriched libraries (AG, AC, and AAG) of P. rex with a modified biotin-streptavidin capture technique. Polymorphism of each locus was assessed in 22 individuals with representation of five populations of P. rex. Number of alleles per locus (A) ranged from two to seven with an average of 4.38. The observed and expected heterozygosities varied from 0.03 to 0.86 and 0.45 to 0.84, respectively. Additionally, among the 13 identified microsatellite markers, 11 of them were successfully amplified in species P. thamnophila, and five of them showed polymorphisms. This study may provide important information for further investigation on the population genetics, introduction, and acclimatization of P. rex and its congeners. |
| 学科领域 | Horticulture |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280041000025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/14111 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Pan-Hui,Yu, Wen-Bin,Yang, Jun-Bo,et al. Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)[J]. HORTSCIENCE,2010,45(7):1129-1131. |
| APA | Huang, Pan-Hui,Yu, Wen-Bin,Yang, Jun-Bo,Wang, Hong,&Lu, Lu.(2010).Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort).HORTSCIENCE,45(7),1129-1131. |
| MLA | Huang, Pan-Hui,et al."Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)".HORTSCIENCE 45.7(2010):1129-1131. |
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